Conger eel (Conger myriaster) is one of the common fish in the East China Sea and other coastal waters, with over fishing and pollution in the marine environment, the wild resources of C. myriaster face the risk of depletion. However, there are limit studies on the reproduction and a lack of research on lipid requirements during the development of the ovary of the C. myriaster. Therefore, lipid metabolism of artificially induced ovary development tissue of lipid metabolism was analyzed in different developmental stages of ovary in this study to provide a theoretical basis for artificial reproduction of the C. myriaster. This study provides insights into the lipid metabolism of the C. myriaster during artificially induced ovarian development. The lipid compositions of the liver, ovary, muscle and plasma in distinct stages were determined using ultra-high performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry (UHPLC/Q-Orbitrap MS). The activity of metabolic enzymes in the liver was investigated as well. By analyzing the lipid components of liver, ovary, muscle, and plasma of C. myriaster at different developmental stages, 68, 27, 63 and 212 lipid markers were identified in the liver, muscle, ovary and plasma, mainly including phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC), triglycerides (TG) and sphingomyelin (SM). Lipids such as TG, diglyceride (DG) and ceramide (Cer) in the four tissues showed different trends during developments. In liver metabolism, the concentration of fatty acid syntheses, liver lipase and lipoprotein lipase showed a process of first increasing and then decreasing. The process was mainly involved in the metabolism of triglycerides, phospholipids and fatty acids. Studies showed that the triglycerides and phospholipids in the main tissues of the C. myriaster had changed to varying degrees during the development of the ovary, and the changes in the activities of lipid metabolism-related enzymes also corresponded to the characteristics of lipid metabolism. This experiment initially explored the lipid mobilization of main tissues during the ovary development of the C. myriaster to provide a basic reference for the artificial reproduction and sustainable development of the C. myriaster.