首页 > 过刊浏览>2018年第42卷第12期 >1967-1976. DOI:10.11964/jfc.20180211184
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高通量测序法分析两株益生菌对凡纳滨对虾肠道菌群结构的影响
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天津师范大学生命科学学院,天津师范大学生命科学学院,天津师范大学生命科学学院,天津师范大学生命科学学院,天津师范大学生命科学学院

基金项目:

国家自然科学基金（31472299）；天津市自然科学基金（15JCZDJC33800）；天津市人才发展特殊支持计划高层次创新创业团队项目（ITTFRS2017007）；天津市高等学校创新团队建设规划（TD13-5076）；国家“八六三”高技术研究发展计划（2012AA092205，2012AA10A401）；国家“九七三”重点基础研究项目（2012CB114405）


Analysis of the characteristics of Litopenaeus vannamei intestinal microflora after being fed with two probiotics using high-throughput sequencing method
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Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Science,Tianjin Normal University,Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Science,Tianjin Normal University,Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Science,Tianjin Normal University,Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Science,Tianjin Normal University,Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Science,Tianjin Normal University

Fund Project:

National Key Technology R & D Program of China (2012 AA 092205, 2012 AA 10 A 401); National Basic Research Program of China (2012 CB 114405);Tianjin Developm- ent Program for Innovation and Entrepreneurship（06202-52K16001）.

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    摘要:

    为分析荚膜红细菌和蜡样芽孢杆菌2株益生菌对凡纳滨对虾肠道菌群结构的影响，本实验对凡纳滨对虾进行为期30 d的养殖饲喂实验，饲喂后期利用高通量测序凡纳滨对虾肠道微生物的16S rDNA V4区，来分析不同益生菌饲喂后凡纳滨对虾肠道菌群的结构特征，并结合对虾体质量增加率和攻毒后累计死亡率的宏观指标来进行分析。结果显示：①空白组（CK）、荚膜红细菌组（Rc）和蜡样芽孢杆菌组（Bc）样品OTU范围为374~506，其中CK组对虾肠道菌群OTU数量最低，饲喂益生菌后的2组对虾肠道菌群中OTU数量相对较高；②在门分类水平上，3组的变形菌门数均为最多，CK组主要为变形菌门和少量拟杆菌门，Bc组主要有变形菌门、拟杆菌门、无壁细菌门、厚壁细菌门和假单胞菌，Rc组主要是变形菌门、拟杆菌门、厚壁菌门、酸杆菌门、放线细菌、梭杆菌门和蓝细菌；③稀释曲线和Shannon指数结果可见，CK组样品物种丰度和复杂度最低，且Bc组样品丰度和复杂度相对较高；④PCA分析发现，Rc组和CK组样品微生物组成较为接近，结合宏观对虾体质量增加率和攻毒后累计死亡率的结果分析，可见相较于荚膜红细菌，蜡样芽孢杆菌对凡纳滨对虾肠道微生物菌群影响更显著，且益生效果更佳。研究表明，饲喂益生菌可以扩增对虾肠道微生物菌群丰度，并能抑制弧菌属等有害菌群的生长，提高对虾体质量增加率并降低死亡率，从而达到益生效果，其中以饲喂蜡样芽孢杆菌菌株效果更佳。

    Abstract:

    In order to analyze the effect of Rhodobacter capsulatus and Bacillus cereusr on the intestinal microorganism of Litopenaeus vannamei, the experiment was carried out for 30 days in order to study the effect of two probiotics on the culture and feeding of L. vannamei. The 16s rDNA V4 region of intestinal microorganism of L. vannamei was sequenced with high throughput in the later stage of feeding to analyze the structural characteristics of intestinal microflora of L. vannamei fed with different probiotics, and combined with the macroscopical indexes of the growth rate of body mass and the cumulative mortality of the prawn after poisoning. The results:①the range of OTU was 374~506, in which the number of OTU in intestinal tract of the prawn was the lowest in blank group, and the number of OTU in intestinal tract of shrimp was relatively high in two groups after feeding probiotics; ②at the level of phylum classification, the number of Proteobacteria was the highest in three groups, the CK group was mainly Proteobacteria and a small amount of Bacteroidetes, the Bc group was mainly Proteobacteria, Bacteroidetes, Tenericutes, Firmicutes and Gemmatimonadetes, the Rc group was mainly Proteobacteria, Bacteroidetes, Firmicutes, Acidobacteria, Actinobacteria, Fusobacteria and Cyanobacteria; ③the results of rarefaction curve and Shannon index showed that the species abundance and complexity of blank samples were the lowest, and the sample abundance and complexity of B.cereus group were relatively high; ④PCA analysis showed that the microbial composition of the samples of R. capsulatus group and blank group was close. Combined with the results of macroscopical growth rate of shrimp body mass and cumulative mortality after poisoning, it can be seen that the effect of B. cereus on intestinal microflora of L. vannamei is more significant than that of R. capsulatus, and the probiotic effect is better. The results showed that feeding probiotics could augment the abundance of intestinal microflora of L. vannamei, inhibit the growth of harmful bacteria such as Vibrio, increase the growth rate of body mass and reduce the mortality of prawn, so as to achieve the probiotic effect. The effect of feeding B. cereus was better than that of R. capsulatus.

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尚碧娇,左志晗,窦春萌,李文悦,孙金生.高通量测序法分析两株益生菌对凡纳滨对虾肠道菌群结构的影响[J].水产学报,2018,42(12):1967~1976

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  • 收稿日期:2018-02-13
  • 最后修改日期:2018-04-06
  • 录用日期:2018-04-11
  • 在线发布日期: 2018-12-04
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