Recombinant positive clones containing microsatellite sequences of Chinese shrimp , Peneaus chinensis were obtained through rapid screening small -size fractionated genomic libraries with PCR technique. The primers used in PCR were T7/ Sp6 promotor primers located at both sides of multiple clone site of pGEM -3zf (+ ) and STR primers : STR1 : 5’-ATATATATATATAT -3’and STR2:5’-TCTCTCTCTCTCTC -3’ designed according to core repeats motifs of microsatellites. The bacterial suspensions were directly used in PCR, before which a short time period of high temperature (95 ℃) was kept to break bacteria. The recombinant positive clones containing microsatellite squences were obtained by screening and were verified after sequencing. The results show that PCR technique combined with bacterial suspension for rapid screening recombinant positive clones containing microsatellite squences is feasible and this method also has advantages of concenience , quickness , high efficiency and reliable outcome.