The standard technologies and models were applied to guarantee the production and supplment of high quality protein in aquaculture. Reirculating aquaculture systems (RAS) has advantages on the level of intensive use, utilization of water resources, and command of environmental factors, and it was widely used in marine fish aquaculture. However, the higher energy consumption and more serious choice and management in fish. Offshore sea cage system (OSCS) and offshore aquaculture nets (OANPS) take efficiency advantages of natural environment to obtain optimal growth performance and flesh quality. Therefore, it is an efficient way to develop offshore aquaculture, promote the high quality development of fisheries and alleviate the offshore pressure which rely on offshore facilities and equipments. Most of fish were difficult to resist severe winter in northern of China, the ingestion and growth will be sifnificantly influenced even if they can tolerance the low tempreture. Therefore, it is effective that the way of land-sea relay to conduct continuous aquaculture. The method was applied in most of marine fish, which were cultivated in RAS and transport to OSCS and OANPS. Besides, the new aquaculture model of land-sea relay will promote developing, ultilizing natural resourses and expanding culture spaces. Spotted knifejaw(Oplegnathus punctatus) was popular with high economic value and broad prospects. According to previous researchers, transport stress significantly influenced the cortisol and glucose concentration in Ictalurus punctatus, Pelteobagrus fulvidraco, and Thunnus orientalis. Our research group has confirmed that handling stress as the main stressor will influence the physiology function of spotted knifejaw. Meanwhile, spotted knifejaw of OSCS has better growth performance, physiology condition and immunity activity than RAS which were cultured in OANPS. So we predicted that spotted knifejaw will obtain optimal tolerance to stress after transport to OSCS and OANPS. The study compared and explained the physiological response mechanism of spotted knifejaw subjected to transport stress under RAS and OSCS, aiming to explore the response features and mechanisms in physiology of spotted knifejaw during land-sea relay under different two kinds of culture models. Plasma cortisol (Cor), glucose (Glu) concentration, white blood cell numbers (WBC)and red blood cell numbers (RBC), hemoglobin (Hb) content, hematocrit (Hct) were analyzed by the automatic hematology analytical method. Hepatic and muscular glycogen content, hepatic superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), alanine transaminase (ALT), aspartate aminotransferase (AST) activity, malonaldehyde (MDA) content were detected by commercial kits. Hepatocyte apoptosis rate was evaluated by TUNEL technique with a commercial in Fluorescein Tunel Cell (FITC) Apoptosis Detection Kit. Results showed that RBC numbers, Hb content, and Hct in spotted knifejaw under OSCS were significantly higher than RAS before transportation, whereas other parameters indicated no significant different under OSCS and RAS models. During transportation, plasma Cor and Glu concentration, plasma and hepatic ALT and AST, hepatic CAT and GSH-Px activity, MDA content, and hepatocytes apoptosis significant increased under two kinds of models, whereas hepatic glycogen was significant decreased and muscular glycogen and SOD activity had no significant changed during the whole experiment. The rate of change of RBC number, Hb, hepatic MDA and glycogen content, Glu concentration, plasma and hepatic ALT and AST, CAT, GSH-Px and hepatocytes apoptosis rate in spotted knifejaw of OSCS was significantly lower than RAS. The above significant parameters accounted for 70.59% of the total indicaors. This different change in physilogical indicators will recovered after transportation 48 h, while RAS needed 72 h. Simultaneously, spotted knifejaw under OSCS had better physiological performance and stronger anti-stress characteristics than RAS during transportation and recovery period. These findings would offer important references and data support to optimize transportation and model during land-sea relay.