虾夷扇贝AMPK基因家族的鉴定及表达
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Q 786;S 917.4

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国家自然科学基金(32102778);中国博士后科学基金(2021M693024);山东省重点研发计划项目(2021ZLGX03)


Genome-wide identification and expression profiling of the AMPK gene family in Patinopecten yessoensis
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    摘要:

    为了解AMPK基因家族在虾夷扇贝中的特征、进化及生物学功能,实验采用比较基因组学及生物信息学的方法对虾夷扇贝AMPK基因家族进行了鉴定、基因与蛋白结构分析、系统发生分析以及时空表达分析,通过虾夷扇贝亚致死温度胁迫实验,研究了AMPK基因家族在高温胁迫时的表达规律。结果显示,虾夷扇贝基因组中共存在3个AMPK家族基因,即PyAMPKαPyAMPKβPyAMPKγ。时空表达分析发现,PyAMPK的3个家族基因在D型幼虫期之前均呈现较高的表达量,其中PyAMPKα基因在受精卵时期表达量最高,PyAMPKβ基因在囊胚时期表达量最高,PyAMPKγ基因在受精卵和2~8细胞时期的表达量达到最高水平。PyAMPK在虾夷扇贝成体器官中呈现不同的表达模式,其中肾脏表达量最高,其次是鳃。受高温胁迫后,肾脏和鳃中的PyAMPK基因短时间内呈现显著的上调表达,并随着时间延长呈现先上升后下降的趋势。研究表明,AMPK基因家族参与了虾夷扇贝早期胚胎发育过程的能量代谢调节以及机体面对高温胁迫的应激调节过程。本研究有助于理解贝类AMPK基因的功能和进化,并为深入阐明贝类应对高温胁迫时的能量调节机制提供理论基础。

    Abstract:

    As the center of cellular energy regulation in eukaryotic cells, AMPK senses and maintains energy balance in peripheral tissues by modulating various metabolic pathways. It plays a vital role in regulating the body's physiological activities and maintaining the body's homeostasis. Although the AMPK gene family has been extensively studied, systematic identification and analysis of the AMPK gene family in Patinopecten yessoensis are still lacking. In order to understand the characteristics, evolution and biological function of the AMPK gene family in P. yessoensis, we conducted genome-wide identification and functional and evolutionary analysis of the AMPK gene family in P. yessoensis. We also investigated the expression profiling of the AMPK gene family under heat stress. The results showed that three AMPK genes were identified in the genome of P. yessoensis including PyAMPKα, PyAMPKβ and PyAMPKγ, which were necessary subunits to form the AMPK complex. Spatiotemporal expression profiling suggested that three AMPK genes have relatively high expression level before the D-shaped larval stage. The PyAMPKα, PyAMPKβ and PyAMPKγ genes exhibited the highest expression level at the fertilized egg stage, blastocyst stage, and the fertilized egg and 2-8 cell stage, respectively. PyAMPK had distinct expression patterns in various adult tissues with the highest expression in kidney, followed by gill. Furthermore, we found the expression levels of all three AMPK genes in the kidney and gill increased first and then decreased over time in the group under the heat stress. Our study results showed that the AMPK genes not only regulated energy balance at early embryo development in scallops, but also participated in the response to heat stress. This study would benefit for understanding the function and evolution of AMPK gene in molluscs, and laid the foundation for further research on the regulation mechanism in response to heat stress in molluscs.

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吕珍立,黄晓文,孙凤芝,邢强,王静,刘平平,安行健,吕佳.虾夷扇贝AMPK基因家族的鉴定及表达[J].水产学报,2024,48(10):109103

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  • 收稿日期:2022-09-24
  • 最后修改日期:2022-10-26
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  • 在线发布日期: 2024-09-30
  • 出版日期: 2024-10-01