羟丙基二淀粉磷酸酯对虾糜凝胶特性及其蛋白结构的影响
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TS254.4

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国家重点研发计划(2020YFD0900903)


Effects of hydroxypropyl distarch phosphate on the gel properties and protein structure of shrimp surimi
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    摘要:

    为考察羟丙基二淀粉磷酸酯(HPDSP)对中华管鞭虾虾糜凝胶性能的改善效果,在虾糜中分别添加1%、3%、5%、7%和9%的HPDSP,以凝胶强度、质地剖面分析、持水性、凝胶微观结构等为指标,并提取虾糜溶胶、凝胶化虾糜、虾糜凝胶中的肌原纤维蛋白,通过圆二色光谱分析蛋白分子二级结构,探究HPDSP对虾糜凝胶性能的影响及其机理。结果显示,添加HPDSP能提高虾糜凝胶的凝胶强度和持水性,并在添加量为7%时达到最大值;HPDSP使虾糜凝胶a*值和b*值下降,但L*值和白度值除9%添加量组外,均无显著变化;适量HPDSP能提高虾糜凝胶硬度、弹性、黏聚性和咀嚼性,促进虾糜形成更加致密稳定的凝胶网状结构。在虾糜凝胶化过程中,加热引起肌原纤维蛋白的α-螺旋含量降低,β-折叠和无规则卷曲含量升高,同时HPDSP会对肌原纤维蛋白热变性和聚集产生影响,从而改变其二级结构,影响虾糜凝胶的形成。研究表明,在中华管鞭虾虾糜中添加7%的HPDSP能显著改善虾糜凝胶性能,研究结果能为高品质中华管鞭虾虾糜制品的开发提供技术支撑。

    Abstract:

    Solenocera crassicornis, also known as red shrimp, is one of the main marine aquatic products of China. It is much-loved by consumers and producers for its good flavor and rich nutrition. Currently, many types of shrimp are sold in the market, such as fresh shrimp, frozen shrimp, dry shrimp or shrimp surimi products, etc. Surimi is a kind of thermally gelated food that was widely accepted by the public because it is rich in protein and low in fat, and is valued for its unique gelling properties. However, due to the physiological and biochemical characteristics of red shrimp meat, it is difficult to form competitive advantage because of poor gel forming and unstable quality, which greatly restricts the development of shrimp surimi products. It is very important to improve the gel properties of shrimp surimi. Compared to the native starch, modified starch usually has better gelatinization performance and be used as additives to significantly improve the performance of the gel. Hydroxypropyl distarch phosphate (HPDSP) is a modified starch which has attracted much attention for its good filling effect, stable gelation properties and low cost in food industry. In order to investigate the effect of HPDSP on improving the gel properties of S. crassicornis shrimi gel, 1%, 3%, 5%, 7% and 9% addition of HPDSP were respectively added to the shrimp surimi. Using the gel strength, texture profile analysis, water holding capacity (WHC), gel microstructure as the indicators, and combined with the analysis of the secondary structure of myofibril protein from shrimp surimi sol, gelatinized shrimp surimi and surimi gel by circular dichroism spectrum, the effects of HPDSP on the gel performance of shrimp surimi and its mechanism were explored. The results showed that HPDSP can increase the gel strength and WHC of shrimp surimi gel, and reached the maximum value when the addition amount was 7%. Furthermore, HPDSP reduced the a* value and b* value of the shrimp surimi gel, but the L* value and whiteness did not change significantly except for the 9% additive group. An appropriate amount of HPDSP could improve the hardness, springiness, cohesiveness and chewiness, and promote the formation of a denser and stabler gel network structure of the shrimp surimi, which was consistent with the gel strength and WHC. In addition, during the gelatinization of shrimp surimi, heating increased the contents of β-sheet and random coil, but decreased the content of α-helix in myofibrillar protein. At the same time, HPDSP affected the thermal denaturation and aggregation of myofibril protein, thereby changing its secondary structure and affecting the formation of shrimp surimi gel. Overall, adding 7% of HPDSP could significantly enhance the gel properties of shrimp surimil. The results of this study would contribute to the utilization of HPDSP for the functionality of surimi products improvement.

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倪伟,米杰,孙仲麒,杨昌杰,魏华茂,贾茹,杨文鸽,余训新.羟丙基二淀粉磷酸酯对虾糜凝胶特性及其蛋白结构的影响[J].水产学报,2021,45(7):1181~1190

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  • 收稿日期:2021-05-15
  • 最后修改日期:2021-06-11
  • 录用日期:2021-06-15
  • 在线发布日期: 2021-08-04
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