合理选取标志放流鱼的规格是成功监测放流鱼类动态及评估增殖效果的关键步骤。本研究以黄鳍棘鲷为对象，通过2个实验评价标志鱼的适宜规格。实验1对比了4种体长的黄鳍棘鲷(平均体长5、7、11和14 cm)作T型标志后其生长、存活和标志保留情况，并利用逻辑斯蒂回归模型分别分析标志鱼存活率和标志保留率的规格特异性；实验2模拟了不同混合比例下2种体长黄鳍棘鲷标志鱼与不标志鱼的捕捞情况，并利用重抽样方法对捕捞结果作计算机重抽样，分析2种鱼能否充分混合以及标志鱼的规格、比例对捕捞结果的影响。结果显示：①7、11和14 cm标志组的特定生长率与对照组无显著差异；②标志鱼的存活率随体长的增加而增加(5 cm标志组的鱼在标志后7 d内全部死亡，7、11和14 cm标志组的存活率分别为77.5%、92.5%和100.0%)。标志鱼的存活率与初始体长的逻辑斯蒂回归关系式为P=exp(0.099X−6.900)/[1+exp(0.099X−6.900)]；③各组的标志保留率较高(7、11和14 cm标志组的标志保留率分别为97.5%、100.0%和100.0%)，但与体长无明显关系；④模拟捕捞结果与计算机重抽样结果无显著差异，二者均不受鱼体规格的影响，但受标志鱼比例的影响极显著，标志鱼比例越高，其捕获率也越高。综上，建议今后采用T型标志方法开展增殖放流品种(如黄鳍棘鲷)的相关研究时，应综合考虑成本和可接受的误差范围以选择符合研究目的的标志鱼规格，若需标志鱼的存活率高于50.0%，则体长至少为7 cm，高于75.0%则体长至少为8 cm，高于95.0%则体长至少为10 cm。此外，在成本允许的条件下，建议尽可能增加放流鱼类中标志鱼的比例以提高增殖效果评估的代表性，而具体适宜的比例值得深入研究。

The data obtained by tag-recapture method are commonly used to evaluate the fish population dynamics and the effect of stock enhancement. However, to ensure the accuracy of the recapture data, it is necessary to choose the appropriate fish size for tagging. Acanthopagrus latus was employed to conduct two indoor experiments. In the first experiment, the effects of T-bar anchor tags on growth, survival and tag retention of A. latus from four size groups (average body length: 5 cm, 7 cm, 11 cm, and 14 cm, respectively) were examined and the size-dependent effects on survival and tag retention were tested. In the second experiment, different mix ratios of tagged fish to untagged fish from two size groups (average body length: 7 cm and 14 cm, respectively) were set for simulated catching and the catching results were resampled. The differences in catching results between groups were compared, the differences between catching result and resampling result were also compared. The results show that: ① there was no significant difference in the specific growth rate between 7 cm, 11 cm and 14 cm groups. ② the survival rate was size-dependent. All the tagged fish of 5 cm group died in the first week after tagging. The survival rate of other size groups was 77.5%, 92.5% and 100.0%, respectively. The relational expression of logistic regression between body length and the probability of survival was: P=exp(0.099X−6.900)/[1+exp(0.099X−6.900)]. ③ the tag retention rate was high (97.5% in 7 cm group, 100.0% in 11 cm and 14 cm group) but seemed to be unrelated with fish size. ④ there was no significant effect of fish size on the catching result and the resampling result but significant effect of mix ratio of tagged to untagged fish on it. In summary, it is recommended that study objectives and cost should be considered when choosing the appropriate fish size for tagging stocking fishes like A. latus. If the probability of survival needs to be >50.0%, the tagged individuals with body length should be >7 cm. If >75.0%, the body length should be >8 cm. If >95.0%, the body length should be >10 cm. And it is worth further study to know about the appropriate proportion of tagged fish for tagging and releasing.

S931.9

国家重点研发计划(2020YFD0901103)；南方海洋科学与工程广东省实验室(湛江)资助项目(ZJW-2019-06)；公益性行业(农业)科研专项(201403008)