Antarctic fish species have a very long life history and are slow-growing. Age determination of Antarctic fish is more difficult than fish species outside the Antarctic area because of unique biological characteristics. However, age determination of Antarctic fish is the basis of fishery stock assessment. Recently, some calcified structures, such as scales, fin rays, vertebrae and otoliths became the main materials for age determination of Antarctic fish. This review compares different methods and materials of Antarctic fish age determination, as well as analyzes their merits and deficiencies to provide the references for further studying Antarctic fish species. The results showed that, compared to otolith, scales and fin rays were seldom used to age determination of Antarctic fish. Length frequency distribution could not be suitable to age older Antarctic fishes because of the long life history. Otolith weight could erroneously estimate the age of Antarctic fishes because of typical individuals occurring. Because fin rays are easily damaged, vertebrae are difficult to collect, the age of some Antarctic fish species without scales were frequently estimated based on otolith sections and verified by scales as well. Meanwhile, some disadvantages, such as no unified age determination protocol, strong subjectivity and lack of background knowledge of early life history, etc. limit the accuracy and precision of ageing Antarctic fish species. Otolith microchemistry and microstructure will be used extensively for studying early life history of Antarctic fish.