Transposons are movable and extended elements, they are important parts in genome and can affect the genome evolution. Transposable activity is usually regulated by environmental factors. To discuss the influence of environmental stress on the activity of transposons, the zebrafish embryos at early stage were exposed to TCDD, Cu²⁺ and Cd²⁺ for two hours, respectively. Then the embryos were cultured in normal medium until 72 hpf. The total RNA of embryos were extracted and transcribed into cDNA by reverse transcription. Then the transcription activity of 9 transposons with complete gene structures after exposure were compared with that before by real-time quantity fluorescent PCR. The 9 transposons included 5 DNA transposons (Tc-a, Tc-b, Tc-c, Tc-d and Tc-e) and 4 retrotransposons (ZB-ERV-1, ZB-ERV-2, 2 L1-323 and L1-21). The results showed that the transcription activity of 8 transposons treated with TCDD obviously decreased, and that of one significantly increased; the transcription activity of 7 transposons treated with Cu²⁺ obviously increased, and that of 2 were significantly decreased; the transcription activity of 6 transposons treated with Cd²⁺ obviously increased, and that of 3 significantly decreased. Those results suggested that environment factors can significantly affect transposons expression activity, and environment stress could be the important reason for the variation of transposon activity. Those data play a very important role in evaluating and understanding the effect of environment factors on fish genome and transposon activity, and provide reference for further research on biology genome evolutionary mechanism.