An 8-week feeding trial was conducted to evaluate the effects of different dietary pantothenic acid levels on growth performance, feed utilization and serum indices of juvenile Pacific white shrimp (Litopenaeus vannamei). Six isonitrogenous and isolipidic practical diets (41% crude protein and 8% crude lipid) were formulated to supplement with pantothenic acid levels were of (control diet), 50, 100, 150, 300 and 600 mg/kg, respectively. The analyzed values of dietary pantothenic acid are 20.9, 69.3, 99.0, 150.2, 304.4 and 513.6 mg/kg, respectively. Each diet was randomly assigned to 3 replicates of 40 juvenile shrimps (initial weight approximately 0.73±0.12 g). The results indicated that weight gain rate and specific growth rate were not significantly affected by dietary pantothenic acid levels (P>0.05). However, shrimp fed the control diet had lower survival rate than those fed the diets supplemented with pantothenic acid (P<0.05), while there were no significant differences among those supplemented with pantothenic acid. Feed efficiency and protein efficiency rate significantly increased with dietary pantothenic acid level increasing from 20.9 mg/kg to 99.0 mg/kg, then decreased with further increase of dietary pantothenic acid level from 150.2 mg/kg to 513.6 mg/kg. Composition of whole body and muscle in the juvenile L. vannamei were not significantly influenced by the dietary pantothenic acid levels (P>0.05). Shrimp fed control pantothenic acid diet had lower glucose concentration in serum than those fed the other diets, while there were no significant differences among all diets supplemented with pantothenic acid. Shrimp fed the diet containing 150.2 mg/kg pantothenic acid had lower TG in serum than those fed the diets containing 20.9 mg/kg and 513.6 mg/kg. Total protein and cholesterol in serum were not affected by dietary pantothenic acid levels. SOD activity in serum increased significantly with dietary pantothenic acid level increasing from 20.9 mg/kg to 150.2 mg/kg, then decreased significantly with further increase of dietary pantothenic acid level (P<0.05). Shrimp fed the diet containing 99.0 mg/kg pantothenic acid had higher T-AOC than the other diets. Shrimp fed the diet containing 69.3 mg/kg pantothenic acid and control diet had higher level MDA in serum than those fed the other diets, and the lowest MDA in serum occurred at diet containing 150.2 mg/kg pantothenic acid (P<0.05). Based on a two-slope broken-line model between feed efficiency, productive protein value and dietary pantothenic acid levels, the optimal dietary pantothenic acid requirement is estimated to be 113.40 and 119.87 mg/kg for juvenile Pacific white shrimp, respectively.