Aeromonas schubertii had caused snakehead disease with white spots in internal organs, which resulted in economic losses in snakehead industry. In this study, A. schubertii strain WL-4 which was isolated from the snakehead (Channa maculate ♀×C. argus ♂) was analyzed in relation with its physiology and biochemistry characteristics, its growth characteristics and virulence factors, and its susceptibility was tested against 20 antibiotics. Its physiology and biochemistry characteristics were tested by using bacterial biochemical identification instrument and its growth situation was analyzed at different temperatures, salinities and pH values. In comparison with the type strain, A. schubertii ATCC43700, A. schubertii WL-4 strain was almost same as the type strain in major physiological and biological characteristics. Moreover, 16S rRNA gene of A. schubertii WL-4 strain is 99. 9% similar to that in A. schubertii ATCC43700. The results of growth characteristics showed that the WL-4 strain's optimum growth temperature was 30 ℃, optimal salinity was 5 and optimum pH was 7. In addition, the WL-4 strain exhibited virulence to snakeheads (25±5)g in experimental challenges with LC₅₀ being 1.2×10⁶ CFU/mL. The WL-4 strain was positive for hemolytic activity, and elastase and lipase activity by phenotypic determination. Susceptibility tests, which detected the minimum inhibition concentration (MIC) of 20 antibacterial agents by trace double dilution method, showed that the strain WL-4 was resistant to sulfonamides and amide alcohols, and susceptible to enrofloxacin, norfloxacin, doxycycline, neomycin, ampicillin and so on. In conclusion, biological characteristics of the WL-4 strain were consistent with the occurrence of the disease with white spots in snakeheads' internal organs which were caused by A. schubertii. In addition, the results of antibacterial susceptibility tests may have an applied value in the control of A. schubertii infection agents in aquaculture.