Ranaviruses not only infect frogs but can also infect many other aquaculture animals, such as fish, amphibians and reptiles. Here, three aquatic animal cell lines, including Chinese giant salamander thymus cell line (GSTC), Epithelioma papulosum cyprini cell line (EPC) and Xenopus kidney cell line (A6), were infected with two raninvirus strains, Rana grylio virus (RGV) and Andriasda davidianus ranavirus (ADRV), respectively. After the ranavirus infection, the morphological changes in these three cell lines were observed by light microscope. The pathological changes and virus titer were recorded and compared at different time post infection. Microscopic observation showed that A6 cells and EPC cells swelled and ruptured. GSTC cells shrank and gathered together to multilayer. The virus titers of two ranaviruses varied significantly in different cells lines. For RGV, 10^3.6, 10^5.9 and 10^6.6TCID₅₀/mL in A6, EPC and GSTC cells respectively. For ADRV, 10^4.3, 10^5.4 and 10^6.1 TCID₅₀/mL in A6, EPC and GSTC cells, respectively. These results showed that the susceptibilities of the same aquatic animal cell lines to different ranavirues were different. This suggested that GSTC is the most sensitive cell line to the ranaviruses. This study provides valuable information and materials for the further study of the pathogenesis of ranaviruses.