Abstract:To learn more about the enrichment and release characteristics of heavy metals in the feed and the tissues of Eriocheir sinensis, kinetic parameters were gained for biological accumulation and release of two heavy metals Pb and Cd in gill, hepatopancreas and muscle of E. sinensis, by using two-compartment bioconcentration model to carry out the experiment in which the crabs were exposed to the feed with Pb concent of 10.21 mg/kg, 22.01 mg/kg, 40.81 mg/kg, and Cd concent of 1.78 mg/kg, 2.80 mg/kg, 4.48 mg/kg, so as to provide theoretical basis for the distribution, migration and enrichment, and provide guiding significance for safe production eriocheir sinensis for Pb and Cd in E. sinensis. Heavy metal uptake rate constant (k1), extracting rate constant (k2), bioconcentration factor (BCF) and biological half-lives (B1/2), equilibrium concentration of heavy metals in vivo CAmax were obtained by non-linear curve fitting. The results showed:(1) The concent of Pb in crab gill, hepatopancreas and muscle exhibited an apparently positive correlation to the accumulation time as well as the concent. On the forty-eighth day of enrichment, the content of Pb in each tissue reached the maximum, the content in gill was 0.18 mg/kg, 1.14 mg/kg, 1.27 mg/kg and 1.91 mg/kg respectively; Content in hepatopancreas was 1.00 mg/kg, 2.17 mg/kg, 2.33 mg/kg and 3.50 mg/kg respectively; Content in muscle was 0.18 mg/kg, 0.73 mg/kg, 1.00 mg/kg and 1.35 mg/kg respectively; The accumulation of Cd in gill and hepatopancreas presented the similar results to that of Pb.On the forty-eighth day of enrichment, the content of Cd in gill reached the maximum, the content of was 0.026 mg/kg, 0.073 mg/kg, 0.107 mg/kg and 0.154 mg/kg respectively. In addition to the twenty-fourth day of the group C, the content of the hepatopancreas reached the highest,was 1.90 mg/kg; The other 3 groups of experiments, the highest content was reached in the enrichment of forty-eighth days, it was 0.33 mg/kg, 1.05 mg/kg and 1.24 mg/kg respectively. In group C, the content of forty-eighth days decreased, it wsa 1.76 mg/kg. But there was no obvious rule of Cd in muscle. (2) The accumulation and release of Pb and Cd were both slow in tissues and organs of E. sinensis. At steady state the concentration of Pb in crab gill, hepatopancreas and muscle ranged from 1.07 mg/kg to 1.69 mg/kg, 4.87 mg/kg to 4.95 mg/kg, 0.79 mg/kg to 1.28 mg/kg, the concentration of Cd in crab gill, hepatopancreas ranged from 0.06 mg/kg to 0.14 mg/kg, 1.25 mg/kg to 2.66 mg/kg. The bioconcentration factor (BCF) of Pb and Cd in the tissues and organs ranged from 0.03 to 0.48 and 0.03 to 0.87, respectively, indicating that the absorption of Cd was much more higher than that of Pb in E. sinensis. The biological half-lives of Pb and Cd in the tissues and organs (B1/2) ranged from 9 to 67 d and 8 to 48 d, which signified that the release of Cd is significantly slower than that of Pb. (3) Pb and Cd were accumulated selectively in the major organs and tissues in E. sinensis. After feeding with the feed, Pb and Cd were distributed in the same way, namely, hepatopancreas > gills > muscle.