This study was conducted to determine the effects of commercial compound diet and fresh frozen Ammodytes personatus on growth, antioxidant ability and lipid metabolism of hybrid grouper (Epinephelus fuscoguttatus♀×E. lanceolatus♂) juveniles. Ten thousand five hundred and forty-two groupers[initial average weight (191.47±3.70) g] were randomly divided into two groups with three replicates at a stock density of one thousand seven hundred and fifty-seven, and commercial compound diet and fresh frozen A.personatus were allocated to each of two groups of fish for a feeding period of 75 days. The results showed that the weight gain rate (WGR), specific growth rate (SGR), condition factor (CF),feed conversion ratio (FCR) and whole body fat content of hybrid grouper juveniles fed with fresh frozen trash fish group were significantly higher than those of fish fed with commercial compound diet (P<0.05), and the hepatosmatic index (HSI), viscerosomatic index (VSI) were significantly lower than those of commercial compound diet group (P<0.05). But the survival rate (SR) and protein efficiency ratio (PER) had no difference between fresh frozen trash fish group and commercial compound diet group (P>0.05). The contents of total cholesterol (CHO), triglyceride (TG), and low density lipoprotein cholesterol (LDL-C) in serum of commercial diet group were significantly decreased compared with fresh frozen A.personatus group (P<0.05). The activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) in commercial compound diet were significantly higher than those of fresh frozen A. personatus group (P< 0.05). There was an obvious decrease in levels of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase enzyme (CAT) and fatty acid synthase (FAS) (P<0.05), and a significant increase in activity of lipoprotein lipase (LPL) was observed in commercial diet group (P<0.05), while the levels of malic dehydrogenase (MDH) and carnitine palmitoyltransferase-Ⅱ (CPT-Ⅱ) were not impacted by two diets. Under given conditions, the fresh frozen A.personatust is better than commercial compound diet, and commercial compound diet may cause an adverse effect on liver of hybrid grouper juveniles. Therefore, it is necessary to optimize and adjust the nutrient formula of hybrid grouper juveniles by referring to the quality nutrition characteristics of fresh frozen A.personatus.