The Dynamic Energy Budget (DEB) theory is a mechanistic theory on metabolism that captures the flows of energy and matter through out the entire life cycle of an organism. A DEB model of an individual organism describes the rates at which the organism assimilates and utilizes energy for maintenance, growth and reproduction, as a function of the state of the organism and of its environment. The DEB model assumes common physiological processes across species and the only difference is in parameter values. It achieves this through a relatively high degree of abstraction, with the pragmatic consequence that the state variables and core parameters are not directly observable. In this paper, both laboratory and field experiments were designed to collect datasets for parameter estimation of scallop Patinopecten yessoensis. 5 basic parameters, such as shape coefficient (δ_m), Arrhenius temperature (T_A), volume-specific maintenance costs per unit of time ([ρ_M]), volume-specific costs for growth (E_G) and maximum storage density (E_M) were measured and calculated in this paper. And the advantages, disadvantages and precautions of these methods were analyzed. By the method of line regression of shell length and soft tissue wet weight of Patinopecten yessoensis to calculate the shape coefficient; With static method to measure the oxygen consumption rate of scallop at different temperature conditions, and then to calculate the Arrhenius temperature; by starvation meathod to get the parameters of [???], [E_G] and [E_M]. The lab-based starvation experiment was done over 60 days. Changes in body flesh weight were monitored and the oxygen consumption rate was measured. The experiment was finished when the oxygen consumption rate and the dry weight of the soft tissue remained constant. The results showed: (1) The regression relationship between shell length (SL) and soft part wet weight (WW) is WW=0.0118SL^3.4511 (R²=0.9365). According to the formula V=(δ_mL)³, the soft tissue wet weight of the cube root and shell length linear regression, the slope is the shape coefficient. The various estimates by means of weighted regression were highly significant (R²=0.8776, p<0.05). δ_m value was 0.32, which was in the range of reported results for other species of bivalves. (2) T_A was calculated on experiments on oxygen consumption as function of temperature. The mean value of T_A was (4160±767) K. (3) Soft tissue DW and oxygen consumption rate decreased by 56% and 81% respectively over the starvation experiment. Ash free dry flesh weight was proportional to the dry flesh weight, with coefficients of 81.4% and 53.0% respectively at the beginning and late stages of the experiment. [???], [E_G] and [E_M] were estimated to be 25.9 J cm^-3 d^-1, 3160 J cm^-3 and 2030 J cm^-3, respectively. These results have laid the foundation for the construction of the DEB model of scallop.