坛紫菜杂交重组品系的选育与特性分析
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上海海洋大学水产与生命学院,上海海洋大学水产与生命学院

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国家“八六三”高科技研究发展计划(2012AA10A411);国家自然科学基金(31072208);国家海洋局公益专项(201105008,201105023);国家农业科技成果转化资金项目(2013GB2C220537);农业部公益性专项(200903030);上海市科委重点科技攻关项目(10391901100);上海高校水产学一流学科建设项目;福建省科技重大专项(2011NZ0001-1);福建省省长专项基金(2014S1477-10)


Selection and characterization of an improved strain by hybridization recombinant in Pyropia haitanensis
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College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai,College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai

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    摘要:

    坛紫菜杂交重组品系(HR-6)具有叶状体生长前期生长较快,色素蛋白含量高,但成熟较早,生长期短,丝状体的壳孢子放散量较少等特性; 坛紫菜另一个杂交重组品系(HR-5)具有叶状体生长前期生长较慢,色素蛋白含量高,但成熟晚,生长期较长,丝状体的壳孢子放散量多等特性。为改善HR-6品系叶状体成熟较早,壳孢子放散量较少的弱点,本文以HR-6品系为父本,HR-5品系为母本进行种内杂交,从杂合丝状体产生的F1叶状体中选育出综合性状更加优良的品系(WD-7)。在叶状体的快速生长前期(日龄30~45 d),父本品系的绝对生长率为6.68 cm/d,母本品系为3.63 cm/d,WD-7品系为6.67 cm/d; 在叶状体的快速生长中后期(日龄46~60 d),父本品系的绝对生长率降至5.15 cm/d,母本品系则升至7.27 cm/d,而WD-7品系则高达11.54 cm/d。父本品系的叶状体成熟最早,培养35 d左右,大部分个体已成熟; WD-7品系遗传了母本品系成熟较晚的优点,日龄55 d之后,大部分个体才出现成熟。日龄35 d的叶状体平均厚度,WD-7品系为29.87 μm,分别比父、母本品系增加了10%和16%,藻体的韧性明显增强。WD-7品系的壳孢子放散总量为183.42万个/壳,分别是父、母本品系的1.17倍和0.57倍。上述结果证实,WD-7品系不仅遗传了父本品系叶状体生长前期生长较快的特性,同时又遗传了母本品系叶状体成熟晚、生长期长、中后期生长快的优点,壳孢子放散量也比父本品系有所增加,该品系有望被培育成适宜大规模栽培的新品种。

    Abstract:

    In this crossing-experiment,the male parental strain (HR-6) ofPyropia haitanensis was characterized by faster growth speed in early growing stage,higher contents of photosynthetic pigments,but early maturity and shorter growth period in gametophytic blades phase,and low production of conchospores in conchocelis phase.The female parental strain (HR-5) of Pyropia haitanensis was characterized by lower growth speed in early growing stage,higher contents of photosynthetic pigments,later maturity and longer growth period in gametophytic blades phase,and high production of conchospores in conchocelis phase.From the F1 color-sectored blades produced by the heterozygous conchocelies,an improved strain (WD-7) with better comprehensive properties than the parental strains was isolated.During the early growth period (30-45 days) of the blades,the absolute growth rate of the male and female parental strains was 6.68 and 3.63 cm/d,respectively,while that of WD-7 strain was 6.67 cm/d; During the middle growing stage (46-60 days) of the blades,the absolute growth rate of the male and female parental strains was 5.15 and 7.27 cm/d,respectively,while that of WD-7 strain was 11.54 cm/d.The male parental strain matured earlier,and most of the 35-day-old blades matured.While the WD-7 strain matured later which was similar to the female parental strain,a large number of blades matured after being cultured for 55 days.The mean thickness of the 35-day-old blades of WD-7strain was 29.87 μm,increasing by 10% and 16% in contrast with that of the male and the female parental strains,respectively.The total number of conchospores released from WD-7 strain was 183.42×104,which was 1.17-fold and 0.57-fold of that of its male and female parental strains,respectively.The above results confirmed that WD-7 strain inherited the excellent characteristics of faster growing speed in early growing stage from the male parental strain,meanwhile inherited the excellent characteristic of late maturity,long growth period,faster growth speed in the middle growing stage and high production of conchospores from the female parental strain,and may offer an alternative for the Pyropia industry.

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丁洪昌,严兴洪.坛紫菜杂交重组品系的选育与特性分析[J].水产学报,2015,39(9):1359~1367

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  • 收稿日期:2015-04-21
  • 最后修改日期:2015-05-28
  • 录用日期:2015-09-20
  • 在线发布日期: 2015-09-23
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