The crustacean hyperglycemic hormone(CHH) family has been found only in arthropods currently,and plays significant roles in regulation of glucose levels,molting,stress response and a variety of physiological metabolic activities.We selected Esflol(Eriocheir sinensis flotillin-1 like,Esflol) as our object of study afer transcriptome analysis and gene expression profiling,to understand the importance of this family-crustacean hyperglycemic hormone(CHH),how to regulate glucose levels of the E.sinensis.According to the prompting of transcriptiome,a cDNA sequence encoding Esflol was cloned from hepatopancreas of E.sinensis by RACEs,and the results of tissue expression analysis were also obained.The results show that the open reading frame of Esflol consists of 1281 bp,encoding 426 amino acids without signal peptide.Typical domains of SPFH super family and PHB domain.Multi-sequence alignments analysis showed Esflol shared the higest identity with E.sinensis flotillin-1(57%).Tissue distribution of Esflol was detected by quantitative real-time PCR,although strong expression of Esflol was detected in hepatopancreas and muscles,and its expression could also be found in heart,gill,gut,ganglion and stomach,and in lower expression levels of brain.This study lays the foundation for sugar metabolic physiological mechanism research of E.sinensis,and also provides a reference for sugar metabolic physiological mechanism research of other aquaculture crustaceans.