间歇性投喂模式对梭鱼幼鱼消化系统发育及形态行为的影响
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中国水产科学研究院南海水产研究所,中国水产科学研究院南海水产研究所,中国水产科学研究院南海水产研究所,中国水产科学研究院南海水产研究所,中国水产科学研究院南海水产研究所,广东省珠海鲻科鱼类良种场;广东省珠海鲻科鱼类良种场

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广东省科技计划(2012A020602016);广东省海洋渔业科技推广专项(A201101D02)


Effects of intermittent feeding on the development of the digestivesystem and morphological behavior for juveniles of Liza haematocheila
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South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science,,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science,,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science,,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science,,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science,,Guangdong Zhuhai Well-bred Mullets Fish Farm

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    摘要:

    为了探讨梭鱼幼鱼对间歇性投喂模式的补偿生长效应,本实验在水温(19±2)℃,pH 7.2,盐度3.5,含氧量6 mg/L的条件下,对相同日龄(36日龄)、相似大小的梭鱼幼鱼分别投喂1 d 2次、1 d投喂1次、2 d投喂1次、4 d投喂1次和8 d投喂1次(设为S0、S1、S2、S4和S8组),循环投喂21 d,于22 d取样,并测量体质量、体长、体高、头长、肛前长等性状。采用解剖、Bouin'S液固定、石蜡切片、H.E染色和光学显微镜等技术,对不同实验组梭鱼幼鱼的消化器官形态以及组织结构进行了观察,测量食道、胃和肠道上皮层高度、柱状细胞高、壁厚、肌层厚以及食道和肠道杯状细胞的大小,用SPSS19.0软件分析数据,分析间歇性饥饿对其组织学的影响。结果显示:在幼鱼行为上,S0、S1组集群性较强,运动迅速,S4、S8组集群性减弱,受惊吓呈分散分布,且运动性稍差。形态结构方面,S0组个体增长幅度最大,几乎与同期池养育苗的幼鱼生长相同,S1组个体稍小,体质量上与S0组存在显著性差异,S4、S8组均有不同程度的损伤,表现在头大身小,胃体积减小,壁变薄;肠管壁变薄变透明,肠系膜因脂肪基本消失而萎缩。肝体积明显缩小,颜色由原来的粉红色变深红黄色。组织结构方面,S0组间组织变化小,不明显。S1、S2组的黏液细胞显著增多,胃腺细胞发达,但分泌颗粒较小,肠皱壁高度下降,上皮细胞高度显著降低;S0和S1组纹状缘高度无显著变化;S1组到S4组,纹状缘高度渐低甚至不规则;S4、S8组纹状缘高度显著降低。此外,S8组消化道变化最为明显,胃肠黏膜中内分泌颗粒减少,肠道纹状缘有断裂脱落现象,从前肠到后肠柱状细胞的高度均显著降低,杯状细胞体积显著变小,肝细胞体积缩小,脂质空泡数量减少,细胞界限不明显,排列不规则,胰腺泡缩小,排列不规则。实验结果表明,梭鱼幼鱼在应对短期间歇性饥饿时,能相应调节自身的形态组织结构,存在补偿效应,但当间歇性饥饿时间增加到一定时间以后,鱼类消化系统组织结构将会发生一些不可逆的变化。

    Abstract:

    A 22-day feeding trial was conducted to assess the compensatory growth response to different cyclic starvation and refeeding regimes in juvenile Liza haematocheila. The test was performed in the conditions of the water temperature:(19±2)℃, pH:7.2, salinity:3.5, the oxygen content was 6%,the same age(36 days), the similar size, the L. haematocheila juvenile were fed:twice a day,once a day,once 2 days,once 4 days,once 8 days(namely S0, S1, S2, S4, S8), cyclic feeding 20 d, sampling at 22 d. We measured morphological characters about the body weight, body length, body height, head length, length before anal and so on. The experiments were carried out using anatomy, Bonn's solution fixation, paraffin sections, H.E staining, optical microscopy techniques to observe the juvenile barracuda's structure, morphology of the digestive organs in different groups. The esophagus, stomach and intestines cortex height, tall columnar cells, wall thickness, muscle layer thickness and the size of the esophagus and intestinal goblet cells were measured. Using SPSS19.0 software for measurement data,we analysed the impact of intermittent starvation on their histology. The results showed:in terms of juvenile behavior, the cluster of S0 and S1 was strong, swift movement, the cluster of S4 and S8 was weak, when they were frightened, they were in sporadic distribution, and the movement was somewhat less. In terms of morphology, the individuals of S0 had the largest increase, almost with the same period the growth of juvenile fish in the nursery pond, individuals of S1 were smaller than S0, there was a significant difference between the groups in the weight in both S0 and S1; S4, S8 had varying degrees of damage,with a big head and a little body,stomach volume was reduced, wall thinning; intestinal wall thinning transparent, mesenteric fat disappeared because of atrophy. Liver volume was significantly reduced with the color change from the original pink to crimson yellow.In tissue structure, S0 inter-group's tissue has somewhat changed, but it was not obvious. S1, S2 had a significant increase in the mucus cells; the gastric cells developed, but the secretory granules decreased,intestinal folds height decreased significantly and epithelial cell height reduced, the striated border height in S0 and S1 did not change significantly;from S1 to S4,the striated border height gradually became smaller, even irregular, S4, S8 group striated border height reduced significantly. In addition, obvious changes could be found in gastrointestinal mucosa of S8 group with fewer endocrine granule,intestinal brush border broken off, height of columnar cells reduced significantly from the front to the rear of intestine,goblet cells was significantly smaller in volume, liver cell shrinkage, reducing the number of lipid vacuoles, cell boundaries obvious and irregular, pancreatic acinar narrow, irregular arrangement. The results showed that when juvenile L. haematocheila at intermittent short-term starvation,the fish could adjust itself accordingly in tissue structure, the compensation effect existed, but when the intermittent hunger had a certain period of time,and then the digestive structure will display some irreversible phenomenon.

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聂广锋,李加儿,区又君,王鹏飞,温久福,汤清亮.间歇性投喂模式对梭鱼幼鱼消化系统发育及形态行为的影响[J].水产学报,2015,39(12):1837~1851

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  • 收稿日期:2015-03-10
  • 最后修改日期:2015-07-31
  • 录用日期:2015-12-24
  • 在线发布日期: 2015-12-24
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