To investigate the relationship between the developmental capacity and biochemical parameters of egg in Takifugu obscurus,the basic components,phosphatase activity,malate dehydrogenase activity,sialic acid,amino acid content in unfertilized eggs of T.obscurus were measured by means of biochemical analysis,also fatty acids composition were measured by means of GC-MS,and the correlation between components and hatching rates were analysed.Results of experiments showed that activity of acid phosphatase,sialic acid content and the hatching rate were significantly correlated,alkaline phosphatase and hatching rate were significantly negative correlated.C14:0 and hatching rate were significantly negative correlated,and showed a decline trend.ARA,DHA and hatching rates were significantly correlated,and both of them showed a tendency of first increasing and then decreasing with the hatching rate.The content of n-3 fatty and the ratio of DHA and EPA and the ratio of EPA and ARA all had significant correlation with the hatching rate.The results demonstrated that phosphatase,sialic acid,fatty acid and egg hatching rate have correlation,also when the activity of ACP were in the range of 68.045,141.038,the activity of AKP in the range of 2.298-7.241, the content of SA in the range of 9.081-19.973, the content of C14:0 in the range of 0.485-0.658, the content of ARA in the range of 1.371-2.498, the content of DHA in the range of 13.838-18.469,all of them could be the references of which hatching rates were no less than 50%.Accordingly these references could reflect the developing value of T.obscurus' egg in breeding production,which could also be used as reference index for embryo and objective evaluation of the quality of its egg.