To detect Vibrio mimicus' dynamic distribution in infected C.idella, the gene encoding enhanced green fluorescent protein(EGFP)was cloned into the expression plasmid pBAD24 and transformed into the host cells V.mimicus strain 04-14 by electroporation to generate recombinant V.mimicus.The recombinant EGFP was highly expressed under induction with arabinose.The EGFP on the cell surface was also confirmed by fluorescence microscopy and flow cytometric analysis.Under nonselective condition, the stability of recombinant plasmid was 100% in EGFP-labeled V.mimicus after 30-generation time, and there was no significant difference between EGFP-labeled strain and wild strain in morphology, growth characteristics and adhesion ability to EPC cells.C.idella were immersed in water containing EGFP-labeled V.mimicus at the concentration of 5×10⁷ CFU/mL.At least three fish per group were sacrificed at different time post infection, and in different tissues and organs, such as gill, intestine, muscle, head kidney, spleen and liver were sampled separately.EGFP-labeled V.mimicus in different tissues and organs was examined by the signal of fluorescence and the method of plate culture count within 4 days after infection.The green fluorescent signals appeared firstly in the intestine and gill at 4 h after infection, and the number of bacteria reached up to 3.60×10⁸ and 2.36×10⁶ CFU/g, respectively.The number of bacteria in the intestine and gill had no obvious changes from 4 to 10 h post infection, then declined gradually after 12 h, but continued to exist until fish death.Dynamics of bacteria in muscle, head kidney, spleen and liver were similar, the green fluorescent signals in these tissues and organs were found 24 h later after infection, and the number of bacteria increased at first but later decreased from 24 to 85 h.The number of bacteria in muscle, head kidney, spleen and liver reached their peaks 48 h post infection, being 9.58×10⁴, 8.75×10⁴, 1.50×10⁴ and 4.50×10⁴ CFU/g, respectively, but the number of bacteria which was detected in them was lower than that in the intestine.These results suggest that the intestine is the primary target organ for the adhesion, colonization and reproduction of V.mimicus.