In this study,we qualitatively compared and analysed microbial community structure in stomach,pylorus,foregut,midgut,hindgut of wild and farmed silver pomfret(Pampus argenteus),isolated and identified produced protease,amylase,lipase,cellulase strains.The results showed very different structure of flora between each part of the digestive tract,and microflora populations of the entire digestive tract of the wild and farmed silver pomfret were quite different.The digestive tract flora of the wild silver pomfret mainly included Psychrobacter species,Bacillus species,and the digestive tract flora of the farmed silver pomfret mainly included Acinetobacter species,Pseudomonas species,Psychrobacter species.Psychrobacter piscatorii strain VSD503 was the only kind of bacteria,which not only existed in the digestive tract of the wild silver pomfret but also existed in the digestive tract of the breeding silver pomfret,but there are different parts.In the experiments of identification of enzyme-producing bacteria,culturable bacteria in the digestive tract of wild silver pomfret yielded protease by 44%,amylase by 56%,lipase by 11%,cellulase by 56%,of which more than three enzymes strains had 5 kinds,and rich enzyme production.Relative to the wild silver pomfret,culturable bacteria in the digestive trace of breeding silver pomfret mainly produced protease and amylase,producing protease by 70%,producing amylase by 21%,and only Bacillus thuringiensis strain VITGS producing cellulose,no bacteria could produce lipase,and only Bacillus thuringiensis strain VITGS can yield three kind of enzymes,but relatively few amount of enzyme production.Analysis shows that the structure of the digestive tract flora affected the species and the activity of digestive enzymes,so enzyme-producing bacteria in the digestive tract of wild silver pomfret can be used as feed additives for the aquaculture of silver pomfret,which can increase production of silver pomfret.