This paper focused on the high efficient utilization of by-product of Trichiurus haumela.The hydrolyzed ferrous chelate prepared from by-product of T.haumela was prepared and initially classified by the method of gel chromatography,and recovery and molecular weight(MW)were confirmed.The constitutes and content of amino acid of different chelates were investigated using automatic amino acid analyzer,while the amino acid score were also confirmed.The therapeutic effect of different molecular weight chelates in improving iron-deficiency anemia was conducted by IDA model rats.The results showed that the chelation was classified into 4 materials,the molecular weight of which were 35 400,8 720,465 and less than 200 u,respectively.All of these materials belonged to incomplete protein except component Ⅱ.The evaluation results of amino acid model of ideal protein showed that AAS of these 4 materials were 85.31%,88.22%,101.32%,105.83%,and were also high quality protein.Content of Fe differed significantly from each other,the content of Fe of component Ⅰ and Ⅱ were 5.23% and 3.34%,and there was a similarity in Fe content between Ⅲ and Ⅳ(4.22% and 4.58%).The results of rat experiment showed that different chelation did not have the same effect on weight,serum iron,TIBC,liver MDA and SOD,and component Ⅱ showed the optimal effect in anemia improvement compared with FeSO₄.Though component Ⅰ did not possess the significant effect,it also had a certain ability in curing anemia.