Abstract:We evaluated the effects of dietary lipid levels on body index,lipid deposition,blood biochemistry indices and lipid metabolic enzyme activities of Sebastes schlegeli.Juvenile fish[average initial body weight:(7.13±0.03)g]were randomly divided into 5 groups and fed diets containing different lipid levels(3.7%,6.8%,9.6%,13.1% and 15.6%)using fish oil as the lipid source for 60 d.The results showed that the hepatosomatic index(HSI)and viscerosomatic index(VSI)of S.schlegeli increased with increasing dietary lipid levels(P<0.05),while the condition factor(CF)had no significant differences among groups(P<0.05).The crude lipid content of whole body,muscle and liver of S.schlegeli increased with increasing dietary lipid levels(P<0.05).The crude lipid contents of whole body,muscle and liver in the group fed 15.6% lipid were significantly higher than those of the groups fed 3.7% and 6.8% lipid(P<0.05).Dietary lipid had a significant effect on lipid metabolic enzymes(P<0.05).The activities of hepatic glucose-6-phosphate dehydrogenase(G6PDH)and malic dehydrogenase(MDA)decreased with increasing lipid level,but the activities of lipoprotein lipase(HPL)and hepaticlipase(HL)increased(P<0.05).The activities of G6PDH and MDA were significantly lower in the groups fed 15.6% and 13.1% lipid than those of the groups fed 3.7% and 6.8% lipid(P<0.05),but the activities of HPL and HL were significantly higher(P<0.05).The contents of blood triglyceride(TG)and cholesterol(CHO)decreased with increasing dietary lipid levels,but the contents of blood high density lipoprotein cholesterol(HDL-C),glutamic oxaloacetic transaminase(AST)and glutamic pyruvic transaminase(ALT)increased(P<0.05).The contents of blood TG and CHO in the group fed 15.6% lipid were significantly lower than those of the groups fed 3.7% and 6.8% lipid(P<0.05),but the contents of blood HDL-C,AST and ALT were highest.The contents of blood low density lipoprotein cholesterol(LDL-C)initially increased with increasing lipid levels,but then decreased(P<0.05).Results of the above show that dietary lipid level had significant effects on body index,lipid deposition,blood biochemistry indices and lipid metabolic enzyme activities of S.schlegeli,but when dietary lipid level exceeds 9.6%,the lipid deposition in liver would increase,which is bad for health and growth of S.schlegeli.