Abstract:Lipid and fatty acids are among the most vital factors that provide marine fish with structural components and energy resources.The fatty acid reserves of broodstocks profoundly affect the fecundity of spawners as well as the quality of the newly hatched larvae.In order to state the characteristics of lipid and fatty acid accumulation in wild-caught broodstocks of Pampus argenteus,the lipid and fatty acid content in different tissues of wild broodstocks of silver pomfret,Pampus argenteus from Mindong Seazone during the reproductive season were quantitatively investigated with the methods of chloroform-methanol and gas chromatography.The results indicated that there were significant differences in lipid content among gonad,liver and muscle.Mean total lipid levels(% in dry weight)in ovary,testis,liver and muscle were 35.76%,15.11%,22.07% and 22.14%,respectively.Testis displayed the highest proportion of polar lipid/total lipid,while muscle displayed the lowest proportion of polar lipid/total lipid.During the reproductive season,the total lipids and neutral lipid contents in ovary increased significantly with the development of the ovary,while the polar lipid content in muscle decreased significantly with the development of the testis.The contents of 20:5n-3(EPA,2.25-3.87 mg/g),22:6n-3(DHA,6.71-13.03 mg/g)and highly unsaturated fatty acids(HUFAs,17.20-29.64 mg/g)in neutral lipid of ovary were the highest.The contents of EPA(0.38-0.27 mg/g)and DHA(3.12-3.59 mg/g)in polar lipid of testis were the highest.The n-3/n-6 ratio in gonad was significantly higher than those in liver and muscle.The essential fatty acids such as DHA were transferred among the tissues and between different lipid classes of the same tissue.It is therefore suggested that significant differences in total lipid content,total lipid classes and fatty acid contents(mg/g dry mass)were displayed not only among the tissues,but also between different lipid classes of the same tissue.The essential fatty acids were preferentially transferred from neutral lipid in muscle and liver to neutral lipid in ovary with the maturation of ovary.