采用育肥养殖实验和生化分析技术，研究了育肥时间对三疣梭子蟹雌体卵巢发育和营养品质的影响。结果发现，（1）经90 d育肥的雌体卵巢指数显著上升，出肉率和总可食率呈先上升后下降趋势，育肥30～60 d的总可食率达42%以上。（2）育肥时间对肌肉中常规营养成分含量无显著影响，育肥60 d卵巢和肝胰腺中的蛋白质、脂肪和碳水化合物含量最高，水分含量最低；育肥使肝胰腺和卵巢中胆固醇百分含量呈显著下降趋势，育肥后期卵巢和肝胰腺中的磷脂含量显著上升；就不同组织而言，肝胰腺中的甘油三酯含量最高。（3）育肥使卵巢和肝胰腺中的单不饱和脂肪酸呈上升趋势，而多不饱和脂肪酸呈先下降后上升趋势，肌肉中脂肪酸组成无显著变化；整体上，肌肉的脂肪酸营养价值优于肝胰腺和卵巢。（4）育肥早期卵巢中氨基酸含量显著上升，整个育肥过程中肌肉中的甜味氨基酸含量呈显著上升趋势，育肥60 d后卵巢和肌肉中所有必需氨基酸分值均大于100，无限制性氨基酸。研究表明，生殖蜕壳后的三疣梭子蟹雌体适宜育肥时间为60 d，育肥不仅提高了总可食率，且优化了其营养价值。

The swimming crab,Portunus trituberculatus,is a commercially important fisheries resource and mariculture species in East Asian countries.Female crab with fully developed ovary is considered as the highly nutritious seafood.Therefore,the decline in natural stock and increase in market demands have driven substantial aquaculture interests for this crab species since 1990's.However,our previous studies have shown pond-reared females generally have poor ovarian development and lower nutrition value compared to wild females,which leads to low percentage of high quality females with fully developed ovaries from pond culture of P.trituberculatus.Fattening with high quality live food,such as trash fish and clam,has proven to be an effective practice for the improvement of crab ovarian development and nutritional composition.Unfortunately,to date,optimal fattening period is unclear for female P.trituberculatus.The present study was conducted to investigate the effects of fattening period on ovarian development and nutritional quality of female swimming crab by fattening culture and biochemical analysis.The results showed that gonadosomatic index(GSI)increased significantly during the 90-day fattening period while the trend of "low-high-low" was found in muscular yield and total edible yield.The highest total edible yield(more than 42%)was in the females fattened for 30-60 days.Fattening period did not affect the proximate composition of muscle,but the highest protein,lipid and carbohydrate contents,together with the lowest moisture contents in ovary and hepatopancreas,were found in the females with 60 days fattening.The cholesterol percentage(%total lipids)in ovary and hepatopancreas decreased significantly during the course of 0-60 days fattening while phospholipids levels of ovary and hepatopancreas increased significantly during 60-90 days fattening.For the different edible parts,hepatopancreas had the highest triacyglycerol levels.As for the fatty acid composition,the increasing trend was found in mono-unsaturated fatty acids(MUFA)for ovary and hepatopancreas while the trend of "high-low-high" was found in poly-unsaturated fatty acids(PUFA)and highly unsaturated fatty acids(HUFA).Muscle contained significant higher percentage of HUFA than ovary and hepatopancreas.Total amino acids(TAA)of ovary increased dramatically during the early period of fattening while the contents of three sweet amino acids gradually increased during the process of 90-day-fattening.Essential amino acid scores(EAAS)of ovary and muscle were greater than 100 for all essential amino acids(EAA)after females fattened for 60-90 days,which indicated no limited amino acid was found in ovary and muscle.For fattening females,EAA/TAA ratios of ovary and muscle were close to or higher than FAO/WHO/UNU recommended values for healthy food.In conclusion,the fattening would not only improve the total edible yield,but also optimize the nutritional values for female P.trituberculatus.Our results suggested the appropriate fattening period was approximately 60 days for pond-reared females post puberty molting.Those results would not only provide valuable information for the future culture and fattening of female P.trituberculatus,but also facilitate the further study on the nutritional regulation of female quality.

Q 956; S 968.2

国家自然科学基金项目（40806068，41276158）；上海高校水产学一流学科建设项目（沪教科2012-62）；上海市自然科学基金项目（12ZR1413000）；上海市科委工程技术中心能力提升项目（13DZ2280500）；浙江省重大科技专项计划项目（2011C12011）；浙江省农业科技成果转化资金项目（2013-171-72）