The present work was carried out to study the effect of temperature on some metabolic enzyme activities in the female swimming crab(Portunus trituberculatus)during its calcification process in laboratory.Activities of PK,HK and SDH were measured in hepatic,muscular and branchial tissue of the swimming crab at 1,2,8,16 h after molting and the completely hardening stage at different temperatures(18 ℃,22 ℃,26 ℃),respectively.The primary results are recorded as belows:①Compared to moderate temperature(22 ℃),PK activity in muscle of P.trituberculatus was found to be significantly increased during 1-8 h after molting at higher temperature(26 ℃)and lower temperature(18 ℃),indicating that the glycolysis rate increased in muscular tissue of the swimming crab at higher and lower temperatures; PK dynamics was found to be peaked in hepatic and branchial tissues of P.trituberculatus at 1-2 h after molting which showed a greater rate of glycolysis in these two tissues.②HK activity in the hepatic tissue of P.trituberculatus decreased during the calcification process in all treatments.HK activity in the liver at 1-16 h was significantly higher than the activity of calcified status,indicating a gradually decreased utilizing capacity of glucose in liver of P.trituberculatus during the calcification process.However,the branchial HK activities showed an opposite dynamic trend compared with that in hepatic tissue,which projected an increased utilizing capacity of glucose levels in gills of P.trituberculatus during the calcification process.The difference may be related to different capabilities to use glucose in different tissues of the swimming crab during calcification process.③SDH activity in hepatic tissue of P.trituberculatus in the all treatments was found to be gradually increased within 16h after molting,indicating that a gradual strengthening of the citric acid cycle in the liver during calcification process.In addition,SDH dynamics was found to be peaked in muscular and branchial tissues of crab occurring within 8h after molting in the all treatments.The activity of SDH at low temperature(18 ℃)was significantly lower than that at high temperature(26 ℃).The statistics described above showed an aerobic strengthening in musclar and branchial tissues of P.trituberculatus at high temperature compared to the low temperature.In summary,glycolysis process under low temperature conditions did not change significantly,while the citric acid cycle had been significantly weakened during the calcification process.The activity of SDH in muscle and branchial tissues of P.trituberculatus at lower temperature was significantly lower than that at higher temperature,indicating that lower temperature can indeed extend the time of its soft-shell stage after molting.