Dmrt family is one of the oldest developmental gene family, and is important to the embryo organizes normal growth, Spermatogenesisspermatogenesis, and the nervous system and sense organ development. Dmrt2 is one of the members of Dmrt family, were which was cloned in mammals such as human, birds such as Gallus gallus, Aquatic aquatic animals such as Oryzias latipes, are is closely related to gonad Development development and growth. RACE -PCR technique wasere used to clone Dmrt2 gene in Andrias davidianus, 2 026 bp full-length cDNA was obtained, containing 1 581 bp open reading frame(ORF), 58 bp 5′’UTR, 387 bp 3′’UTR, GenBank number is FJ859987. This sequence encoded 526 amino acids, and the amino acid sequence was across the membrane protein, no signal peptide, and the protein positioning in the cytoplasm. The amino acid sequence was analyzed by the Conserved Domain Database(CDD), surveyed that single amino-terminal DM domain is from 93 bp to 146 bp, contains a pattern of conserved zinc chelating residues C2H2C4, super-family pfam00751 and smart003010. Blast DM domain with Homo sapiens mab-3, Gallus G. gallus Dmrt2, Xenopus (Silurana) tropicalis Dmrt2 and Danio rerio mab-3, five amino acid were had variation, include Ile, Arg, Met, Thr, Cys. Phylogenetic analysis indicated that the five species above of Homo sapiens , Gallus G. gallus , Xenopus X. (Silurana) tropicalis and Danio D. rerio were clustered first, that means Dmrt family is highly conserved during evolution, and the five amino acids variation have had little affection effect onto protein functions. RT-PCR was used to detecte detect the expression of Dmrt2 in skin, intestines, gills, muscle, stomach, spermary and kidney of Andrias A. davidianus, it and results showed it is high level expression only in spermary and muscle, which is indicated that the Dmrt2 gene maybe play an important role in gonad development and growth development.