Research was made in this paper on the moisture, lipid content and fatty acid composition of muscle, liver and ovary of adult female Tridentiger trigonocephalus collected from the Yangtze Estuary at Ⅴ stage of ovary development. The results indicated that: (1) The moisture content in muscle, liver and ovary was reduced successively, and the value was significantly different among these three tissues (P<0.05). The total lipid content in these three tissues was (1.32%±0.08%), (7.75%±0.38%), and (26.73%±0.59%), respectively. There was significant difference in total lipid content among these three tissues, and the highest was in ovary, followed by liver, and the lowest was in muscle. (2) The content of SFA in muscle was significant higher than that in liver and ovary. There was significant difference in SFA content among three tissues (P<0.05). About the MUFA, C16:1 content of liver is the highest. The C16:1 content in muscle, liver and ovary were (11.90%±0.71%), (15.28%±2.42%) and (9.49%±0.37%) respectively, and there was significant difference among these three tissues (P<0.05). The content of PUFA, w3PUFA, w6PUFA in ovary was significantly higher than that in muscle and liver (P<0.05), but showed no significant difference between muscle and liver (P>0.05). The total content of EPA and DHA in muscle, liver and ovary were increased successively. The total content of EPA and DHA in these three tissues was (30.17%±1.02%), (30.53%±0.50%) and (34.33%±0.71%), respectively. The content of EPA and DHA in ovary was significantly higher than that in muscle and liver (P<0.05), but showed no significant difference between muscle and liver (P>0.05). The ratio of EPA to DHA (EPA/DHA) in these three tissues was 1.50, 1.75 and 1.59 respectively, and the ratio of total w3 polyunsaturated fatty acids to total w6 polyunsaturated fatty acids (∑w3PUFA/∑w6PUFA) in these three tissues was 6.44, 8.05 and 7.00, respectively. The ratios of EPA/DHA and ∑w3PUFA/∑w6PUFA were highest in liver, followed by ovary, and the lowest was in muscle.