Follistatin related protein (FRP) is an extracellular glycoprotein, involved in various physiological processes such as cell proliferation, migration, tissue remodeling, embryonic development, and cell-cell interaction. However, no FRP gene has been reported from crustaceans. In this study, we isolated FRP gene from mud crab, Scylla paramamosain by RACE (rapid amplification of cDNA ends). And we analyzed the expression of FRP transcripts in different tissues and ovarian development stages by semi-quantitative and quantitative RT-PCR. The obtained cDNA was 1 948 bp, with the 3’UTR of 493bp. The putative peptide of sp-FRP was composed of 484 amino acids, which was most similar to the red flour beetles Tribolium castaneum FRP (50%). Sp-FRP contained a KAZAL-FS domain, an EFh domain, and two immunoglobulin domains, suggesting that FRP may act as a scaffold protein linking the TGF-β family and other unknown proteins to modulate their functions. The phylogenetic analysis was consistent with traditional classification. Quantitative PCR showed that the expression of FRP was significantly higher in brain and thoracic ganglia than in ovary. In addition, the expression of FRP transcript was different during ovarian development, and much higher at undeveloped stage of ovary. FRP may play a suppression role in the ovarian development of the mud crab.