The aim of this study was to investigate the effects of starvation and refeeding on tissue and serum metabolic enzyme activities and glycogen contents of Oplegnathus fasciatus with an average weight of(10.0±1.0) g.Five experimental groups were designed,i.e.fed twice daily for 30 d(S0,control group),starved for 3d and refed for 27 d(S3),starved for 6d and refed for 24 d(S6),starved for 9d and refed for21 d(S9),starved for 12 d and refed for 18 d(S12).The samples(serum,liver and muscle)were collected at the initial experiment,after starvation and refeeding,and the alkaline phosphatase(AKP),acid phosphatase(ACP),glutamicpyruvic transaminase(GPT),glutamicoxaloacetic transaminase(GOT)activities and glycogen contents of tissues were analyzed.The results showed that,serum and liver glycogen contents were significantly affected by the starvation and refeeding,and serum glycogen(except S12 group)and liver glycogen contents were reduced significantly as a result of starvation,while the liver glycogen contents returned to the level of control group after refeeding.However,only a little effect on the muscle glycogen contents was found during the starvation and refeeding.During the period of experiment,the AKP and GPT activities of serum and liver were significantly affected by starvation,and after refeeding,both enzymes activities returned to the levels of control group.However,during the whole experimental period,the effects of starvation and refeeding on muscle AKP,ACP,GPT and GOT activities were very little.In conclusion,the serum glycogen content with a level of (2.65±0.33)-(3.70±0.36) mmol/L was essential to maintain the stabilization of body metabolism.The main metabolic enzymes in serum and liver were very important in maintaining the basic metabolism of O.fasciatus under the condition of starvation.