Starvation is a situation undergone and tolerated by many fish species in the natural environment. Starvation is also an important environment factor for fish and it can affect fish growth, metabolism, behavior, reproduction, development and survival, as well as causes fish to infect diseases even to death. In order to estimate effects of starvation on growth, physiological and biochemical parameters of grass carp, fish with body weight(31.86±1.47) g were randomly divided into two groups(the starvation group, the control group), the starvation group was starved for 15, 30, 45, 60 d and the control group fed diet which met the nutritional requirements of grass carp.The results showed, with the prolonged time of starvation, hepatosomatic index and viserosomatic index decreased significantly compared with the control group (P<0.05). During the starvation, protein and lipid contents in muscle markedly declined, and moisture content increased (P<0.05). The contents of total protein, triacylglycerol, total cholesterol, ALT and ALB in the serum fluctuated in wave-like curves and decreased as a whole during starvation. As one of the important indexes reflecting the growth of fishes, RNA︰DNA ratio in muscle had no significant difference, but there were significant differences in the hepatopancreas tissue compared with the control group (P<0.05). In order to estimate the effects of starvation on the protein metabolism, the activity of glutamate dehydrogenase was determined in serum, muscular and hepatopancreas tissues. The results showed that the activity of glutamate dehydrogenase was increased with the time increase of the starvation in the hepatopancreas, not in the other tissues.Glutamic dehydrogenase in serum and hepatopancreas was regarded as an operative index of protein metabolization compared with those in muscle tissue. To sum up, the tested fish used the substances(lipid and protein) of body which fluctuated in wave-like curves during 60-day starvation period.