The karyotype of Verasper variegatus was studied through the chromosome preparations obtained from head kidney by the method of injecting PHA and colchicines.Metaphase chromosomes were analyzed by means of C-banding,Ag-NORs and G-banding.Experiment results showed that the diploid chromosome number of V.variegatus was 46(2n=46t,NF=46).There was no visible evidence of sex chromosome and satellite chromosome.C-bands of V.variegatus were located mainly in the centromere regions of chromosomes except the No.19 and 22 chromosomes,and the 19th pair of chromosomes showed positive C-bands in the terminal region while the whole 22th pair of chromosomes bore positive C-band thoroughly.The C-banding heretochromatin accounts for 30% of the total chromosome surface in V.variegatus.Silver staining study showed that V.variegatus bore a pair of Ag-NORs which was located at the end of long arm of the 2nd chromosome.A total of 59 positive G-bands(38 dark,21 light)were recorded in 23 pairs of chromosomes.These results would provide basic data for the genomic research and biodiversity conservation of V.variegatus.