In the present study,the promoter sequence of largemouth bass POU1F1 was cloned which is 1 629 bp in length,predicted the existence of four octamer transcription factor 1(Oct-1)binding sites,one homeobox transcription factors sites,two cAMPresponsive element binding proteins sites and a TATA box.Using directly sequencing,two nucleotides mutations were identified which were located at the -18 and -183 sites,and only two haplotypes were identified(haplotype A and haplotype B).The different genotype individuals and the mutant alleles in stochastic population(126 individuals)were examined.The frequency of A haplotype and B haplotype was 0.246 and 0.754 respectively.The population correlation analysis showed that the AA and AB genotypes have significantly correlated with BB genotype individuals’ weight,body length,full length,body depth and body width(P<0.05),but there is no significant correlation between AA genotype individuals and AB genotype individuals.Identified growth traits markers for growth traits could be implemented through marker-assisted selection(MAS),with the aim of producing rapidly growing largemouth bass.