Abstract:In the present study the combined effects of ozone(O3)sterilization and modified atmosphere packaging on the quality of Sinonovaculla constricta Lamark were assessed by physicochemical and microbiological methods. S.constricta Lamark were sterilized by ozone(1 mg/L)and packaged by modified atmosphere,then stored at (0±0.5) ℃.There are six groups in the experiment:low oxygen MAP(60% CO2+10%O2+30% N2),non-oxygen MAP(60% CO2+40% N2),air packaging,low oxygen MAP(60% CO2+10% O2+30% N2)+O3,non-oxygen MAP(60% CO2+40% N2)+O3,and air packaging+O3.The microbiological analysis showed that the total viable counts(TVC)for air packaging group and air packaging+O3 group reached 1.76×107 cfu/g and 1.14×107 cfu/g on day 4 and 8 respectively,while the TVC for low oxygen MAP group and non oxygen MAP group reached 1.87×107 cfu/g and 1.03×107 cfu/g respectively on day 10,exceeding the microbiological acceptability limit.The results indicated that the combination of low oxygen MAP and O3 could extend the shelf-life of S.constricta Lamark up to 12 days,while the combination of non-oxygen MAP and O3 could extend the shelf-life up to 14 days.TVB-N,K value,sensory evaluation,pH changes for each group were also associated with the storage time(P<0.05),TVB-N,K value,sensory score and pH value for MAP+O3 group showed better effects than single MAP or ozone group.The preservation effects of nonoxygen MAP(60% CO2+40% N2)+O3 group were better than other groups.TVB-N,K value,and sensory score reached 14.952 mg N/100 g,55% and 5,respectively,at the end of shelf-life(14 days),which indicated a moderately fresh or acceptable status of samples.Thus,it was suggested that the combination of ozone sterilization and modified atmosphere packaging could effectively extend the shelf-life of S.constricta Lamark during refrigerated storage.