Amino acid composition in muscle,liver and ovary of the wild and cultured Paralichthys olivaceus broodstock at stages Ⅲ-Ⅳ and Ⅴ was determined by biochemical methods,to ascertain their effects on the reproductive performance.The results indicated that the total amino acid concentration of muscle,liver and ovary of both the wild and the cultured broodstock is as follows:muscle>ovary>liver;There were no significant differences in most amino acid concentration of muscle between the wild and the cultured broodstock.The muscle of the wild and the cultured broodstock have constant amino acid composition with development of the ovary except Methionine and Histidine; The most amino acid concentration in liver of the cultured broodstock were higher than the samephased wild broodstock,and the amino acid concentration in liver of both the wild and the cultured broodstock increased with the development; There were no significant differences of total amino acid concentration and essential amino acid concentration in ovary between the wild broodstock and the samephased cultured broodstock,but Methionine concentration of the wild broodstock was higher than the cultured one.The amino acid concentration in different tissues of the wild and the cultured broodstock increased with the development especially Arginine,Methionine,Histidine.Arginine,Methionine,Histidine were presumed to induce spawning differences between the wild broodstock and the cultured one,so further researches are needed.