三疣梭子蟹快速生长品系核心种质有效群体含量
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国家“八六三”高技术研究发展计划(2006AA10A406);国家自然科学基金项目(30871933);青岛市科技计划项目(07-2-3-5-jch)


Effective population size of rapid growth line core collection in Portunus trituberculatus
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    摘要:

    三疣梭子蟹是我国海水养殖的重要种类之一,良种选育核心种质的繁衍与更新是制约品种选育成功的技术瓶颈。根据Doyle和Talbot提出的计算有效群体含量的计算公式,对2008年三疣梭子蟹快速生长品系核心育种群有效群体含量进行估算,得出实际群体有效含量为213,实际近交系数为0.002 3,其近交系数较小,近交衰退控制在较低的水平。但三疣梭子蟹保种数量和留种方式仍然困扰育种群体控制,因此,借鉴畜禽的保种模式,从留种方式、雌雄比例、保种数量等方面对三疣梭子蟹快速生长品系核心基础群进行保种研究。结果表明,采用随机留种,雌雄1∶1比例交尾,雌雄数量各131只,此保种模式既能保证三疣梭子蟹快速生长品系核心基础群遗传多样性处于较高水平,使其具有较大的选育潜力,又能使保种成本维持在一个合理水平。此研究模式也为理论保种模式,需要在以后的实际群体选育过程中逐步完善。

    Abstract:

    According to the formulas of effective population size,which were brought forward by Doyle and Talbot,we estimated the effective population size(Ne)for rapid growth line fundamental stock in Portunus trituberculatus.The real Ne was 213,which corresponded to a rate of inbreeding(F)of 0.23%.Results indicated that F is relatively small,and inbreeding depression was in a low level,however,the cost of conservation was too high,it did not meet the conservation requirements.In light of breed conservation model in livestock and poultry,we investigated rapid growth line fundamental stock in P.trituberculatus on methods of selecting parents and ratio of dams and sires and the size of conservation population.We made the rules as follows:random selecting parents,the ratio of dams and sires is 1∶1,and the number of dams and sires is 131,respectively.In this situation,genetic diversity of rapid growth line fundamental stock is preferably rich,which satisfies selective breeding,at the same time the cost of breed conservation is economical.This is only a theoretical model of breed conservation,and it needs the process of gradual improvement of breeding populations in the future.

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任宪云,高保全,刘萍,韩智科,李健.三疣梭子蟹快速生长品系核心种质有效群体含量[J].水产学报,2011,35(4):509~514

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  • 收稿日期:2010-05-08
  • 最后修改日期:2010-09-30
  • 录用日期:2010-10-08
  • 在线发布日期: 2011-04-12
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