In order to assess the potential applications of hybrid F₁ between Pseudosciaena crocea ♀ and Nibea albiflora ♂ in the genetic breeding and the basic research,the inheritance and segregation patterns of AFLP markers in the offspring of two hybrid families(HF1 and HF2)were investigated.Totally,478 and 446 fragments were detected in two pairs of parents by using 8 selective primer combinations,respectively.In the parents of HF1,the amplified fragments included 215 female parentspecific bands(FSB),165 male parentspecific bands(MSB)and 98 mutual bands(MuB).All of these fragments were transmitted to the hybrid offspring.Among these fragments,121(56.3%)FSB,115(69.7%)MSB and 93(94.9%)MuB were transmitted to all the offspring,while the other loci were segregated in the offspring.The level of polymorphism of the FSB was comparable to that in the intraspecific hybrid F₁.There was no significant difference between the average frequency of the segregated FSB and that of the segregated MSB.The inheritance and segregation patterns of AFLP markers in HF2 were similar to those in HF1,except that the proportion of segregated loci in HF2 was lower than that in HF1 and 2 nonparental bands were detected in HF2.In addition,a relative high proportion of loci showing distorted segregation was found in both HF1 and HF2.These results suggested that the newly hatched hybrid fries between P.crocea ♀ and N.albiflora ♂ contained the genome of both parents,and the phenomenon of uniparental gene elimination was not obvious.The findings would serve as the basis for further development and management of the hybrids.