Abstract:In order to provide firsthand scientific data and to utilize domestic cultured puffer fish safely, four tissues(skin, muscle,liver and gonad) of four cultured pufferfish species (Takifugu rubripes, Takifugu fasciatus, Takifugu flavidus, Takifugu bimaculatus) collected from five coastal provinces(Liaoning, Hebei, Shandong, Jiangsu and Fujian) in China were monitored quantitatively by mouse method after grouping and concentration. The skin, muscle and liver groups of T.rubripes from Shandong Province and several ovaries were measured by enzymelinked immunosorbent assay(ELISA) at same time. The results indicated that the average TTX content in each group of skin, muscle and liver of the four species specimens were ‘nontoxic’ in both of the mouse assay (less than 0.5 μg/g) and ELISA (less than 0.8 μg/g), and all the testes have no detectable TTX . However, a moderately toxic ovary sample was still detected. The annual variation of the average TTX content in groups of the muscle, liver and skin of cultured pufferfish can be observed. The results of the mouse method and ELISA method have no significant difference. It is confirmed that the skin, muscle and testis of the collected cultured pufferfish can be used with safety. The livers of the four species of puffer fish could be safely consumed as a delicious and nutritive food. The market size of cultured pufferfish was small, but still toxic. It is necessary to prevent the edible parts contamination.