Grass carp(mean body weight 19.46±0.04 g) were first fed for 60 days by three modes(mode A, grass carp were fed basal diet without chitosan; mode B, grass carp were fed experimental diet, supplemented with 0.5% chitosan in basal diet; mode C, grass carp were first fed experimental diet and then basal diet at an interval of 15 days) and then starved for 15 days. Growth index, nitrogen oxide(NO) content and lysozyme(LSZ) activity in headkidney(HK),spleen,hepatopancreas and serum were measured to investigate effects of feeding modes of chitosan on prevention of starvation of grass carp. The results showed that length gain and weight gain of grass carp at the end of 60 days feeding were significantly higher in modes B and C than those of the control(（P<0.05) and were the similar variation(（P＞0.05) after 15 days starvation, regardless of feeding or starvation. NO content,in HK and hepatopancreas, and LSZ activity, in HK and serum, were significantly higher in starvation treat in mode A. On the contrary, NO content ,except in HK, and LSZ activity or NO content, except in spleen, and LSZ activity, except in hepatopancreas, were significantly lower(（P<0.05) in starvation treat or had no statistical significance(（P＞0.05) between starvation and feeding treats in modes B and C.Obviously, starvation caused various influence in different tissues and effects caused by different oral administration treats were dissimilar on prevention of starvation of grass carp. Comparatively, modes B and C had an effective resistance to starvation stress, but there was no statistical significance in both of them. Given economical efficiency and practicability, chitosan by incontinuous feeding will be suggested to promote growth and lighten the stress caused by starvation.