In order to build base populations for selective breeding, ninety families in nine groups were constructed using a full diallel cross design with parents of Pinctada fucata from three different places ［Beihai (B), Xuwen (X) and Sanya (S)］. Comparisons of the breeding value and general evaluation among families were studied after 60 days culture in outdoor pond. The results showed that the Kung breeding values of shell length were over 20 in eight families (BX2, BX9, BB4, BB5, BB8, BS9, XS3 and SB8). The family XS3 had the largest breeding values in shell length and shell width and comprehensive value, whereas XX3 had the smallest ones. The overall values of BB4 and XS3 were over one. General combining abilities (GCA) in shell length for Sanya, Xuwen and Beihai parents were 17.40, 17.45 and 18.27, respectively while GCAs in shell height were 14.74, 14.79 and 15.54, respectively. Specific combining abilities (SCA) in shell length were 17.75, 18.27 and 18.12 for Sanya(♂)Xuwen(♀), SanyaBeihai and XuwenBeihai matings, respectively while SCAs in shell height were 14.98, 15.51 and 15.46, respectively. Average heteroses in shell length were 1.41, 0.96 and 0.67 for SanyaXuwen, SanyaBeihai and XuwenBeihai matings, respectively while average heteroses in shell height were 1.13, 1.60 and 1.19, respectively. This study suggested that the parents from Beihai are preferable.