Abstract:For the better understanding about the mechanism of lipid metabolism at the molecular level in grass carp Ctenopharyngodon idellas, the partial cDNA sequence of lipoprotein lipase (LPL) gene was cloned (GenBank accession number FJ612596), and its expression in different organs, adipocytes, as well as the influence of fasting and refeeding on it in hepatopancrease were studied by means of semi-quantitative (SQ-RT-PCR . The results showed that the obtained cDNA sequence was 360 bp in length, and the homology is 70%-89% with other species. LPL gene is widely expressed in hepatopancreas, muscle, heart, gill, intrapaneal fat body (IPF),mature adipocytes and stromal vascular fraction (SVF) cells , with the highest expression level in IPF, lowest one in gill among the organs (P<0.05), and higher level in mature adipocytes, compared to SVF cells (P<0.05). On the other hand, LPL gene expression level significantly increased in hepatopancreas after 48 h fasting and retrieved to the same level as 0 h after 12 h refeeding (P<0.05). It could be concluded that LPL gene is wildely expressed in various organs and at the initiation and final differentiation stage of adipocyte, regulated by the energy expenditure status, which implies its important role in lipid metabolism in grass carp. The further study on the modification mechanism of LPL gene is required in fish.