以新型蛋白源（大豆浓缩蛋白、脱酚棉籽蛋白、喷雾干燥血球蛋白粉等）和脱脂鱼粉为原料，通过调节饲料氨基酸水平，配制出5种等氮等能的饲料D0、D17、D35、D52和D69，养殖星斑川鲽，初始体重为（75.6±0.18） g。其中D0（添加58%脱脂鱼粉）为对照组，D17、D35、D52和D69分别为新型蛋白源替代配方中17%、35%、52%和69%的鱼粉用量。每组饲料设3个平行，共养殖60 d。结果表明：对照组(D0)增重率(WGR)、蛋白质效率(PER)、特定生长率(SGR)分别为141.84%、229.88%和1.47%/d。与D0组相比，D69组WGR、PER和SGR显著降低（P<0.05），分别为107.77%、186.37%和1.22%/d ，D17、D35和D52组与D0组均无显著性差异(P>0.05)。对照组(D0)饲料系数(FCR)为0.80，D69组FCR为0.98，差异显著(P<0.05)；D17、D35和D52组与D0组FCR无显著差异(P>0.05)。随新型蛋白源替代鱼粉量的增加，鱼体消化道指数(DTI)逐渐增大，D0组为2.01%，显著小于D69组(P<0.05)。各组间的肥满度(CF)无显著性差异(P>0.05)。全鱼或肌肉中蛋白质和脂肪含量各组间均无显著差异(P>0.05)。全鱼中灰分含量随鱼粉替代量的增加呈增大趋势；红细胞数目、白细胞数目、血红蛋白含量及血清蛋白含量在各组间无显著差异(P>0.05)，但血细胞比容和溶菌酶活力随新型蛋白源替代鱼粉量的增加呈不同程度的降低。本试验条件下，在星斑川鲽幼鱼配合饲料生产中可用新型蛋白源适当替代鱼粉而不影响养殖对象的生长。

Starry flounder (Platichthys stellatus) is the large carnivorous fish and demands diet with high level protein. This experiment was carried out to study the effect of replacing fishmeal with new protein sources in diets of juvenile starry flounder ［initial weight, (75.6±0.18) g］. Five isonitrogenous and isoenergetic diets (D0, D17, D35, D52 and D69) were formulated by regulating amino acid level with new protein resources (soy protein concentrate, dephenolized cottonseed protein, spraydried blood meal, etc). D0 with 58% defatted fishmeal as the control group, fishmeal was replaced by new protein sources at four levels of 17%, 35%, 52%, 69%. Five groups in triplicate were divided randomly for the 60day experimental period. At the end of the experiment, growth performance, body composition and partial immunological parameters were studied. The results show that weight growth rate(WGR), protein efficiency ratio (PER) and specific growth rate (SGR) of D0 are 141.84%，229.88%and 1.47%/d，respectively, no significant differences occurred among D17, D35 and D52 (P>0.05), but D69 is significantly low at 107.77%,186.37% and 1.22%/d (P<0.05), respectively；Feed conversion ratio (FCR) of D0 is 0.80 and not significant to D17, D35 and D52(P>0.05), but D69 is 0.98 and higher than the other four groups obviously(P<0.05)；Digestive tube index(DTI) tends to enhance with increasing level of new protein sources，D0 is 2.01% , significantly lower than D69(P<0.05); Condition factors (CF) has no significant difference (P>0.05) in five groups. There are no significant differences (P>0.05) in the contents of protein and fat in whole fish and muscle，while the mineral content in whole fish increases with the replacement of fishmeal；erythrocyte, leukocyte，hemoglobin and serum protein have no significant difference(P>0.05) among groups，but increase of new protein sources has some negative impact on hematocrit(HCT) and lysozyme activity. These results show clearly that dietary fishmeal levels can be considerably reduced without any adverse effect on growth performances for juvenile starry flounder. Digestive enzyme activities, amino acid and the utilization of minerals of juvenile starry flounder are the target of research at present.

国家重点行业公益项目（农业部，nyhyzx07-046）；国家科技部农转资金项目（03EFN213700155）;山东省水生动物营养与饲料泰山学者岗位经费资助