Increasing economical concerns regarding the use of fish meal in diets for shrimp have led to the development of replacement strategies where plant protein sources have received ample attention. Corn gluten meal, being one of the available plant protein sources, which is obtained as a byproduct from corn, has a high protein level, and it is low in fiber, rich in vitamins B and E and is known to contain no antinutritional factors. In this study, the corn gluten meal as a dietary protein for juvenile Litopenaeus vannamei was evaluated. Four kinds of isocaloric, isonitrogenous diets were formulated. The control diet used fish meal, soybean meal, meat meal and peanut meal as the protein sources, and other three diets contained 5%, 10% and 15% of corn gluten meal respectively (replacing 8.6%, 17.2% and 25.8% of fish meal respectively). The juvenile shrimps with initial weight (0.013 6±0.001 0) g were fed in aquarium indoor for 45 d at 28 ℃. Effects of partial replacement of fish meal by corn gluten meal on growth, daily ration and nutrient ingredients in muscles of Litopenaeus vannamei were determined. The results showed that since dietary corn gluten meal level up to 10% did not significantly affect the mean daily ration（P>0.05）, and 5% treatment had the best growth parameters. Its weight gain (WG), length gain (LG) and feed conversion ratio (FCR) was 2 542.8%, 155.1% and 1.58 respectively. WG was significantly higher than that of the control（P<0.05）, LG and FCR were not significantly different from those of the control（P>0.05）. The growth parameters of 10% treatment were not significantly different from those of the control（P>0.05）, but those of 15% treatment were significantly lower than those of the control（P<0.05）. There were no significant differences in content of moisture, crude protein, crude lipid and crude ash in muscles of Litopenaeus vannamei among treatments(P>0.05), and the total amino acids （TAA）, essential amino acids （TEAA）, delicious amino acids（DAA） and essential amino acids index （EAAI） in muscles of Litopenaeus vannamei in corn gluten meal treatments were also not significantly different from those of the control（P>0.05）. 10% corn gluten meal in the feed for tested shrimps was suggested.