以大菱鲆脾脏为材料，构建cDNA文库，通过对文库克隆的序列测定和初步生物信息学分析，共获得3 656个大菱鲆脾脏表达序列标签（EST）。经与GenBank数据库的序列比对后发现，1 891个EST代表了149个已知基因，其余1 765个EST为未知序列。这149个基因大致可分为6类：9个为细胞结构类（6.0%）；26个为代谢类（17.5%）；45个为细胞防御/免疫类（30.2%）；43个为基因/蛋白质表达类（28.9%）；16个为细胞信号转导/细胞交流类（10.7%）；10个无法明确分类（6.7%）。鉴定的与免疫抗病相关重要基因主要有：CC/CXC趋化因子；主要组织相容性复合体（MHC）class I α；C₃补体；天然杀伤细胞增强因子（NKEF）；胸腺素β-4/β-12；干扰素诱导蛋白Gig1；白介素8受体；STAT 4；热激蛋白70/90等。

A cDNA library was constructed from turbot, Scophthalmus maximus, spleen by unidirectional cloning. A total of 3 656 ESTs from the library were sequenced and compared with sequences in the GenBank database. 1 765 clones (48.3%) appeared to be completely unknown and are likely to represent newly described genes, whereas 1 891 clones (51.7%) were identified based on matches to sequences in the database. These identified clones were derived from at least 149 genes which were categorized into six categories: 9 in cell structure/motility (6.0%), 26 in metabolism (17.5%), 45 in cell defense/immunity (30.2%), 43 in gene/protein expression (28.9%), 16 in cell signal transduction/communication (10.7%), and 10 genes lacking enough information to be classified (6.7%). Immune related cDNAs identified from the spleen were: CC/CXC chemokine, MHC class Iα, complement component C1/C₃, natural killer cell enhancing factor (NKEF), thymosin beta4/ beta12, interferon inducible protein Gig1, ILB4 receptor, STAT4, and hot shock protein 70/90.

国家“八六三”高技术研究发展计划（2006AA10A402）；国家自然科学基金（30671623）；山东省泰山学者工程专项资助