The aim of this study was to assess the effects of adding astaxanthin(100 mg/kg)， canthaxanthin(100 mg/kg) and mixed pigment(50 mg/kg astaxanthin+50 mg/kg canthaxanthin) on muscle pigmentation and total antioxidant capacity of liver in rainbow trout with an initial weight of (56.60±0.63) g. After 60 days feeding, results showed that there were no significant differences of weight gain, feed conversion ratio, muscle proximate composition component , water loss rate and muscle percentage of rainbow trout among groups(P> 0.05). The Salmo Fan score, redness, astaxanthin content of muscle, and carotenoids content of serum in rainbow trout fed diets supplemented with pigments, were higher than that of control group(P< 0.05). SalmoFan score and redness of rainbow trout of astaxanthin group were 26.25 and 18.40, higher than the canthaxanthin group(22.38，14.13) and the mixed pigment group(24.00, 15.70)(P< 0.05). The muscle astaxanthin content of astaxanthin group was 4.75 mg/kg on 30th day and 6.45 mg/kg on 60th day, and both were higher than that of mixed pigment group(3.87 mg/kg, 5.48 mg/kg)(P< 0.05). Fish of astaxanthin group had the highest serum carotenoids content, higher than that of mixed pigment group, the canthaxanthin group. There was no significant difference in total antioxidant capacity of liver among the three pigmentsupplemented groups(2.39 U/mg, 2.26 U/mg, 2.39 U/mg), which was higher than that of control group(2.03 U/mg)(P< 0.05). Results above showed that adding 100 mg/kg astaxanthin, canthaxanthin or mixed pigment(50 mg/kg astaxanthin+50 mg/kg canthaxanthin) in feed could improve the muscle colour and total antioxidant capacity of rainbow trout. Astaxanthin, mixed pigment(50 mg/kg astaxanthin+50 mg/kg canthaxanthin) had better pigmentation than canthaxanthin, and was suggested to be apply in rainbow trout culture.