Aeromonas caviae is a pathogenic bacterium that can infect various farmed fishes, and cause high mortality. Thus, a rapid and sensitive technique is necessary for the detection of the bacteria to prevent further economic losses. For rapid and simple examination, an immunochromatographic lateral flow assay system (GICA) was developed. In GICA, the rabbit antiA. caviae antibody applied to nitrocellose membrane acts as a capture reagent for the target analyte in a sample, and the monoclonal antibody against A. caviae conjugated with colloidal gold acts as signal generator. Sodium citrate was used as reducer to donate electrons to the positively charged gold ions in solution and colloidal gold particles was produced. The size of colloidal gold particles was checked by a transmission electron microscope (TEM), and the TEM images showed the average diameter of colloidal gold particles was almost the same size: approximately 20.0 nm in diameter. We produced monoclonal antibody against A. cavia by hybridoma technology. After cloning, three strains of hybridoma named 0E10，1C4 and 1F10 were obtained. An absorbing pad, often paper, is attached to notrocellose membrane. On the nitrocellose membrane，rabbit anti A. caviae antibody was used as a capture antibody at the test line (T) and goat antimouse IgG antibody was used as the capture antibody at the control line (C). A conjugate pad which was attached to the notrocellose membrane contains gold particles conjugated with 1F10 monoclonal antibody specific to the analyte being detected. A sample pad, usually glass fiber, is attached to the conjugate pad. During detection, the liquid sample migrates by capillary diffusion through the conjugate pad, rehydrating the gold conjugate and allowing the interaction of the sample analyte with the conjugate. The complex of gold conjugate and analyte then moves onto the notrocellose membrane and migrates towards the test line (T), where it becomes immobilized and produces a distinct signal in the form of a sharp red line. A second line, a control line(C), was also formed on the membrane by excess gold conjugate, indicating the test is complete. The preliminary feasibility study of this method was described as follows:the sensitivity of the GICA test strip towards A. cavia was high with a detection limit of 1×10⁶ CFU/mL, The specificity of the assay is 100% with no crossreaction being observed with thirteen bacteria of aquaculture pathogens such as A. hydrophila, A. sobria, Vibrio alginolyticus etc., Accurate reading time needed for confirmation of the assay can be completed in 5 min with a liquid sample of 100 μL, The GICA test strip is stable at room temperature for 6 months or more (data not shown). This study indicated that the GICA test strip assay system provided high sensitivity and specificity for the detection of A. cavia at low concentration. The assay is rapid, simple, cheap, and does not require any sophisticated equipment. Thus, the GICA test strip will be a useful technique for rapid diagnosis of A. cavia infection.