三角帆蚌三种群F1遗传差异的RAPD分析

doi:10.3724/SP.J.00001

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三角帆蚌三种群F1遗传差异的RAPD分析
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                        10.3724/SP.J.00001
                    
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基金项目:上海市重点学科建设项目

RAPD analysis on genetic variance of F1 of Hyriopsis cumingii from three populations

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With the random amplified polymorphic DNA (RAPD) analysis method, forty tenbase arbitrary primers were used to study genetic variance of 9 F1 stocks produced from hybriding three geographical populations of Hyriopsis cumingii from Dongting Lake(DD), Poyang Lake(PP) and Taihu Lake(TT). The amplified results of thirteen primers were stably and clearly endowed with abundant stock polymorphy, and each primer could gain 2-17 fragments (200-3000 bp) in the 40 primers. Proportion of polymorphy was from 55% to 100%. AMOVA of 9 stocks indicated that 7.15% genetic variance was from intrastock and 92.85% from interstock. Fixation index (Fst), the three lower were DP and PD(0.013)，TP and TT(0.020) and TP and TD(0.026)，the 4 higher were as follows: PT and DT(0.165)>TT and PT(0.153) >〖JP〗TD and PT (0.150)> DD and PT(0.137). Inter stock gene diversity (π) was as follows:TP(0.335)>TD(0.317)>TT(0.308)>PD(0.300)>DP(0.298)>PP(0.297)>DT (0.291)>PT(0.291)>DD(0.269).

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李家乐,董志国,郑汉丰.三角帆蚌三种群F1遗传差异的RAPD分析[J].水产学报,2007,31(6):848~852

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收稿日期:2007-11-16
最后修改日期:2007-11-16
录用日期:2007-11-16
在线发布日期: 2007-11-16
出版日期: 2007-11-16

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