Purification and immune analysis of lipovitellin from Xiphophorus helleri
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摘要: 采用Sephacryl S300凝胶过滤层析柱和HiTrap Q阴离子交换柱从卵黄生成期的雌性剑尾鱼（Xiphophorus helleri）卵巢组织提纯了卵黄脂磷蛋白(Lv)。已确定被纯化的剑尾鱼Lv在NativePAGE（4%~7.5%）电泳中分子量为530 kD左右。NativePAGE（4%~7.5%）电泳后的凝胶分别进行糖蛋白染色、脂蛋白染色及磷蛋白染色。结果表明，剑尾鱼Lv是一种富含糖、脂、磷的蛋白。用纯化的剑尾鱼Lv免疫大白鼠获得鼠源多克隆抗血清。用免疫双扩散的方法测定Lv抗血清的效价为l∶32。Westernblotting检测显示抗血清的特异性较好；卵黄蛋白原（Vtg）和Lv抗血清之间存在明显的免疫交叉反应，表明Vtg和Lv两者具有相同的免疫原性。免疫双扩散检测表明，抗Lv血清表现出明显的雌性特异性和种的特异性。
Abstract: Lipovitellin（Lv）was purified from ovaries of mature female swordtai1 fish（Xiphophorus helleri）by gel filtration with Sephacryl S300 HR and anion exchange chromatography with HiTrap Q. The results indicated that the purified Lv appeared approximately 530 kD in native polyacrylamide gel electrophoresis(PAGE).Lv was characterized as a phospholipoglycoprotein by NativePAGE and staining of gels for carbohydrates, lipids and phosphorus. Polyclonal antibodies against the purified Lv from swordtai1 fish were produced in the mice. Double immunodiffusion showed that the determination of the titre for antiLv sera was l∶32. Westernblotting analyses demonstrated that antiLv sera have specificity and Lv has common antigenicity with vitellogenin(Vtg) while purified Vtg and Lv had crossreacted obviously with the antiLv sera. Double immunodiffusion showed that antiLv sera have sexspecificity and speciesspecificity.
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温茹淑.剑尾鱼卵黄脂磷蛋白的纯化及免疫分析[J].水产学报,2007,31(5):647~654
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收稿日期:2007-09-04
最后修改日期:2007-09-04
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在线发布日期: 2007-09-05
出版日期: 2007-09-15
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