酶解植物蛋白对建鲤生长、肝胰脏和肠道发育及消化酶活性的影响
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国家自然科学基金


Effects of vegetable protein enzymatic hydrolysates on growth, hepatopancreas and intestine development, digestive enzymes of Jian carp cyprinus carpio Jian
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    摘要:

    试验考察了酶解植物蛋白对建鲤生长性能、肝胰脏和肠道发育及消化酶活性的影响。体质量为(9.18±0.21)g的建鲤900尾,随机分成6个处理组,每组3个重复,每个重复50尾,分别饲喂酶解植物蛋白替代相应完整植物蛋白0、20%、40%、60%、80%和100%的试验饵料,试验期为60 d。结果表明,酶解蛋白替代完整蛋白40%时,生长速度最快,饵料系数最低,摄饵量、蛋白和灰分沉积率最高;替代比例在60%以下时,肠重和肠皱襞高度、肠道脂肪酶、碱性磷酸酶、Na+, K+-ATP酶的活性随着替代比例的上升而显著增加;替代比例超过60%以后,生长速度显著降低、饵料系数升高,肠道酶活、皱襞高度、蛋白沉积率下降,而肝胰脏谷草转氨酶活性、血氨浓度和脂肪沉积率升高(P<0.05)。 结果表明,适宜比例的酶解植物蛋白能促进建鲤生长、肝胰脏和肠道发育,提高饲料利用率。

    Abstract:

    900 carps with an average initial weight of 9.18±0.21 g, were divided into 6 groups, feeding diets which were formulated with vegetable protein enzymatic hydrolysates (VPEH) 0%、20%、40%、60%、80%、100% of intact protein respectively for 60d. The results indicated that when the VPEH in diet protein is 40%, the weight gain, feed intake, protein and ash productive values were higher than other groups, the feed conversion ration was lower. Intestinal weight and folds height, lipase, alkaline phosphatase and Na+,K+-ATPase activities enhanced with VPEH levels increasing, until to 60%(P<0.05). When VPEH was over 60%, the weight gain、protein and ash productive values, enzymes activities and folds height of intestine decreased(P<0.05), while the glutamine-oxaloacetate transaminase in hepatopancrease、ammonia level in plasma and lipid productive values increased (P<0.05). It suggested that VPEH could promote Jian carp cyprinus carpio Jian growth, hepatopancreas and intestine development, increase digestive enzymes activities.

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冯琳,杨义,周小秋,姜俊,刘扬.酶解植物蛋白对建鲤生长、肝胰脏和肠道发育及消化酶活性的影响[J].水产学报,2008,32(4):608~613

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  • 收稿日期:2007-11-19
  • 最后修改日期:2007-11-27
  • 录用日期:2008-05-28
  • 在线发布日期: 2008-07-07
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