人工养殖不同年龄史氏鲟的血液学参数
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中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室

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国家高技术研究发展计划(863计划)资助(2004AA603110);国家“十五”重点科技攻关计划资助(2004BA526B0114)


Hematological parameters of different age groups of cultured Acipenser schrenckii
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East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China

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    摘要:

    测定了5个年龄组人工养殖史氏鲟的血液学参数,其中红细胞(RBC)、红细胞比积(HCT)、血红蛋白(HGB)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、白细胞(WBC)等随年龄的增长而上升,WBC、HCT5年龄组与1~4龄各年龄组间有显著和极显著差异(P〈0.05);HGB、MCH、MCHC相邻组间无显著性差异;平均血红细胞体积(MCV)、血栓细胞(PLT)、血红蛋白含量分布宽度(HDW)等1~4龄变化不大,仅在5龄MCV、PLT有较显著的上升,而HDW有显著的下降;红细胞体积分布宽度(RDW)则保持相对稳定,各年龄组间无显著差异;平均血栓细胞体积(MPV)变化不显著,随年龄增长呈下降趋势。在白细胞分类计数DLC值变化中,白细胞中各类细胞比例依次为淋巴细胞(Lym)〉嗜碱性粒细胞(Baso)〉嗜中性粒细胞(Neut)〉单核细胞(Mon),随着年龄的增长,Lym的含量逐渐下降,而Baso、Neut和Mon均呈上升趋势。

    Abstract:

    Using hematology analyzer, hematological parameters of 5 age groups ( 1 - 5 years old) of cultured Amur sturgeon, Acipenser schrenckii, were studied. Blood samples were collected using 5 mL medical injectors from the artery or vein near the anal fin of the fish, and the samples were put into tubes with anticoagulant for examination. For each age group, 5 individuals of fish were sampled. Before blood sampling, fish was immobilized using MS-222 at the concentration of 200 mg? L^-1. Examination data were analyzed using software STATISTICA version 6.0 ( Statsoft, Inc. ), and using one-way ANOVA to test the significance of group differences, using LSD for multi comparison, and using GRM for the correlative analysis. The examination results showed that red blood cell (RBC), haematocrit (HCT), hemoglobin ( HGB ), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell count (WBC) increased with the age. WBC and HCT of 5 years old group were significantly higher than that of 1 - 4 year-old groups ( P 〈 0.05 ), however, there was no significant difference of HGB, MCH and MCHC among all groups. Among 1 - 4 year-old groups, there was no significant difference in mean corpuscular volume (MCV), platelet (PLT) count and hemoglobin distribution width (HDW), but the MCV and PLT of 5 years old group increased significantly and HDW reduced significantly. RDW did not change much with the age, but mean platelet volume (MPV) slightly reduced with the age. For the change of differential leukocyte count (DLC), the proportional order of the number of different kinds of cells in the leucocyte indicated lymphocyte (Lym) 〉 basophil (Baso) 〉 neutrophil (Neut) 〉 monocyte (Mon). With the increase of age and Lym reduced, however, Baso and Mono increased. Comparison with other fish species, HCT and RBC of Amur sturgeon were lower than that of most freshwater fish species. MCV of culaued Amur sturgeon was lower than those of wild ones, but MCHC of culaued Amur sturgeon was much higher than that of wild ones. This result is similar to the test of rainbow trout and tilapia. Lym number of culaued Amur sturgeon was high, so was that of the most fish species. Similarly, Baso number of culaued Amur sturgeon was high also, just below the Lym number. This is quite different from that of most freshwater fish species. MCV, PLT and HDW of 5 years old culaued Amur sturgeon were significantly different from those of 1 - 4 year-old groups. This result may imply that these parameters may be related to the age of maturation because 5 years old culaued Amur sturgeon reached maturity.

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章龙珍.人工养殖不同年龄史氏鲟的血液学参数[J].水产学报,2006,30(1):

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  • 收稿日期:2014-03-09
  • 最后修改日期:2014-03-09
  • 录用日期:2014-03-09
  • 在线发布日期: 2014-03-09
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